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MAP2 antibody

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133110
Anti-MAP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133110
Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133110
Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133110
Anti-MAP2 antibody used in Western Blot (WB). GTX133110

Cat No. GTX133110

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

199 kDa. ( Note )

Positive Control

mouse brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Mouse MAP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

microtubule-associated protein 2,G1-397-34,MAP-2,Mtap-2,Mtap2,repro4

Cellular Localization

Cytoplasm, cytoskeleton,Cell projection, dendrite

Database

Research Area

DATA IMAGES
Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133110

GTX133110 ICC/IF Image

MAP2 antibody detects MAP2 protein at by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP2 protein stained by MAP2 antibody (GTX133110) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MAP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133110

GTX133110 IHC-Fr Image

MAP2 antibody detects MAP2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MAP2 protein stained by MAP2 antibody (GTX133110) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133110

GTX133110 ICC/IF Image

MAP2 antibody detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Grenn: MAP2 protein stained by MAP2 antibody (GTX133110) diluted at 1:500.
Red (Top right): Dendrites, stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:1000.
Red (Bottom right): Axons, stained by Tau antibody (GTX49353) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133110

GTX133110 ICC/IF Image

MAP2 antibody detects MAP2 protein at by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP2 protein stained by MAP2 antibody (GTX133110) diluted at 1:500.
Red: Tau, stained by Tau antibody (GTX49353) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MAP2 antibody used in Western Blot (WB). GTX133110

GTX133110 WB Image

Mouse tissue extract (10 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with MAP2 antibody (GTX133110) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169