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MAP2 antibody

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133110
Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133110
Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133110
Anti-MAP2 antibody used in Western Blot (WB). GTX133110
Anti-MAP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133110

Cat. No. GTX133110

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MAP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

199 kDa. ( Note )

Positive Control

mouse brain

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse MAP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886831

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule-associated protein 2 , G1-397-34 , MAP-2 , Mtap-2 , Mtap2 , repro4

Cellular Localization

Cytoplasm, cytoskeleton,Cell projection, dendrite

Database

Research Area

DATA IMAGES

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133110

GTX133110 ICC/IF Image

MAP2 antibody detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Grenn: MAP2 protein stained by MAP2 antibody (GTX133110) diluted at 1:500.
Red (Top right): Dendrites, stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:1000.
Red (Bottom right): Axons, stained by Tau antibody (GTX49353) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133110

GTX133110 ICC/IF Image

MAP2 antibody detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP2 stained by MAP2 antibody (GTX133110) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133110

GTX133110 ICC/IF Image

MAP2 antibody detects MAP2 protein at by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP2 protein stained by MAP2 antibody (GTX133110) diluted at 1:500.
Red: Tau, stained by Tau antibody (GTX49353) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-MAP2 antibody used in Western Blot (WB). GTX133110

GTX133110 WB Image

Mouse tissue extract (10 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MAP2 antibody (GTX133110) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MAP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133110

GTX133110 IHC-Fr Image

MAP2 antibody detects MAP2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MAP2 protein stained by MAP2 antibody (GTX133110) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169