50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Store at -80ºC. Product is stable for at least 6-12 months.
0.1mg/ml(Please refer to the vial label for the specific concentration.)
Baculovirus (Sf9 insect cells)
Purity was assessed by SDS-PAGE (≥90%) and by HPLC.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
mitogenactivated protein kinaseactivated protein kinase3, MAPKAPK3, mitogenactivated protein kinaseactivated protein kinase 3, mitogen-activated protein kinase-activated protein kinase 3, MAPKAPK
MAPKAPK3 has a single potential SH3-binding site in the proline-rich N terminus, a putative ATP-binding site, 2 MAP kinase phosphorylation site motifs, and a putative nuclear localization signal. It shares 72% nucleotide and 75% amino acid identity with MAPKAPK2 (1). MAPKAPK3 was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses (2). This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation (3). MAPKAPK3 is uniquely poised to support luteal maturation through the phosphorylation and activation of the nuclear transcription factor CREB (4).