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MC1 Receptor antibody [C2C3], C-term

Anti-MC1 Receptor antibody [C2C3], C-term used in Western Blot (WB). GTX108190
Anti-MC1 Receptor antibody [C2C3], C-term used in Western Blot (WB). GTX108190
Anti-MC1 Receptor antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108190
Anti-MC1 Receptor antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108190

Cat. No. GTX108190

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MC1 Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Cat, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human MC1 Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950893

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

melanocortin 1 receptor , CMM5 , MSH-R , SHEP2

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MC1 Receptor antibody [C2C3], C-term used in Western Blot (WB). GTX108190

GTX108190 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [C2C3], C-term (GTX108190) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MC1 Receptor antibody [C2C3], C-term used in Western Blot (WB). GTX108190

GTX108190 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [C2C3], C-term (GTX108190) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MC1 Receptor antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108190

GTX108190 IHC-P Image

MC1 Receptor antibody [C2C3], C-term detects MC1R protein at membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
MC1 Receptor antibody [C2C3], C-term (GTX108190) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MC1 Receptor antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108190

GTX108190 IHC-P Image

MC1 Receptor antibody [C2C3], C-term detects MC1R protein at cytosol and membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
MC1 Receptor antibody [C2C3], C-term (GTX108190) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Liu Y et al. J Headache Pain 2024; 25 (1) : 28 LncRNA Anxa10-203 enhances Mc1r?mRNA stability to promote neuropathic pain by recruiting DHX30 in the trigeminal ganglion.
Chen SJ et al. Free Radic Biol Med 2021; 173 : 151-169 The anti-melanogenic effects of 3-O-ethyl ascorbic acid via Nrf2-mediated α-MSH inhibition in UVA-irradiated keratinocytes and autophagy induction in melanocytes.
Hseu YC et al. Redox Biol 2021; 44 : 102007 The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways.
Application : WB
Reactivity : Human
Siming Fu et al. Oxid Med Cell Longev 2020; 2020 : 8864100 Activation of the Melanocortin-1 Receptor by NDP-MSH Attenuates Oxidative Stress and Neuronal Apoptosis through PI3K/Akt/Nrf2 Pathway after Intracerebral Hemorrhage in Mice.
Application : WB, IHC-Fr
Reactivity : Mouse
Wu X et al. J Neuroinflammation 2019; 16 (1) : 192 NDP-MSH binding melanocortin-1 receptor ameliorates neuroinflammation and BBB disruption through CREB/Nr4a1/NF-庥B pathway after intracerebral hemorrhage in mice.
Application : ICC/IF
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169