MCM4 antibody immunoprecipitates MCM4 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM4 protein by 4 μg MCM4 antibody (GTX109740)
7.5 % SDS-PAGE
The immunoprecipitated MCM4 protein was detected by MCM4 antibody (GTX109740) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM4(GTX109740) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using MCM4(GTX109740) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody (GTX109740) diluted at 1:1000.

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM4 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

MCM4 antibody detects MCM4 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
MCM4 antibody (GTX109740) dilution: 1:5000

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using MCM4 antibody (GTX109740) at 1:300 dilution.

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX109740 diluted at 1:1000