Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (GTX110278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 40 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal (GTX110278)
7.5 % SDS-PAGE
The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal (GTX110278) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM7(GTX110278) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using MCM7(GTX110278) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min