GeneTex
United States (US)

MDH2 antibody

KO/KD
GTX105870_39862_20180119_WB_KO_watermark_19011506_327.jpg
GTX105870_39862_WB_R_lung_19011506_508.jpg
GTX105870_39862_IHC_19011506_261.jpg
GTX105870_39862_WB_M_brain_19011506_950.jpg
GTX105870_39862_20150206_IHC_M_19011506_141.jpg
GTX105870_43055_20171220_ICC_IF_19011506_209.jpg
GTX105870_39862_20150206_IHC_M_2_19011506_626.jpg
GTX105870_43054_20171208_WB_19011506_347.jpg

Cat No. GTX105870

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat lung

Predict Reactivity

Zebrafish, Cat, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MDH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

M-MDH antibody, MDH antibody, MGC:3559 antibody, MOR1 antibody, MDH2 antibody, malate dehydrogenase, mitochondrial antibody, malate dehydrogenase 2, NAD (mitochondrial) antibody

Cellular Localization

Mitochondrion matrix

Background

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105870_39862_20180119_WB_KO_watermark_19011506_327.jpg

GTX105870 WB Image

KO/KD

Wild-type (WT) and MDH2 knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody (GTX105870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX105870_39862_WB_R_lung_19011506_508.jpg

GTX105870 WB Image

MDH2 antibody detects MDH2 protein by western blot analysis.
A. 50 µg rat lung lysate/extract
10% SDS-PAGE
MDH2 antibody (GTX105870) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105870_39862_IHC_19011506_261.jpg

GTX105870 IHC-P Image

MDH2 antibody detects MDH2 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
MDH2 antibody (GTX105870) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105870_39862_WB_M_brain_19011506_950.jpg

GTX105870 WB Image

Sample (20 µg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX105870 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105870_39862_20150206_IHC_M_19011506_141.jpg

GTX105870 IHC-P Image

MDH2 antibody detects MDH2 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MDH2 antibody (GTX105870) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105870_43055_20171220_ICC_IF_19011506_209.jpg

GTX105870 ICC/IF Image

MDH2 antibody detects MDH2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

GTX105870_39862_20150206_IHC_M_2_19011506_626.jpg

GTX105870 IHC-P Image

MDH2 antibody detects MDH2 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
MDH2 antibody (GTX105870) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105870_43054_20171208_WB_19011506_347.jpg

GTX105870 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody (GTX105870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169