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MDH2 antibody

KO/KD Orthogonal
Anti-MDH2 antibody used in Western Blot (WB). GTX105870
Anti-MDH2 antibody used in Western Blot (WB). GTX105870
Anti-MDH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105870
Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870
Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870
Anti-MDH2 antibody used in Western Blot (WB). GTX105870
Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870
Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870
Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870
Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870
Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870
Anti-MDH2 antibody used in Western Blot (WB). GTX105870
Anti-MDH2 antibody used in Western Blot (WB). GTX105870

Cat. No. GTX105870

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MDH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Mouse brain , rat lung , 293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Cat, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MDH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950902

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

malate dehydrogenase 2 , EIEE51 , M-MDH , MDH , MGC:3559 , MOR1

Cellular Localization

Mitochondrion matrix

Background

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MDH2 antibody used in Western Blot (WB). GTX105870

GTX105870 WB Image

KO/KD

Wild-type (WT) and MDH2 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody (GTX105870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MDH2 antibody used in Western Blot (WB). GTX105870

GTX105870 WB Image

Sample (20 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX105870 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MDH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105870

GTX105870 ICC/IF Image

MDH2 antibody detects MDH2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870

GTX105870 IHC-P Image

MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870

GTX105870 IHC-P Image

MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MDH2 antibody used in Western Blot (WB). GTX105870

GTX105870 WB Image

MDH2 antibody detects MDH2 protein by western blot analysis.
A. 50 μg rat lung lysate/extract
10% SDS-PAGE
MDH2 antibody (GTX105870) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870

GTX105870 IHC-P Image

MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:1000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870

GTX105870 IHC-P Image

MDH2 antibody detects MDH2 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
MDH2 antibody (GTX105870) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870

GTX105870 IHC-P Image

MDH2 antibody detects MDH2 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
MDH2 antibody (GTX105870) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870

GTX105870 IHC-P Image

MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:1000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Anti-MDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105870

GTX105870 IHC-P Image

MDH2 antibody detects MDH2 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
MDH2 antibody (GTX105870) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MDH2 antibody used in Western Blot (WB). GTX105870

GTX105870 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody (GTX105870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MDH2 antibody used in Western Blot (WB). GTX105870

GTX105870 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with MDH2 antibody (GTX105870) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Marco Rosina et al. Cell Metab 2022; 34 (4) : 533-548.e12 Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue.
Application : WB
Reactivity : Mouse
Yi-Wen Lo et al. Journal of Cellular and Molecular Medicine 2015; 19 (4) : 744-59 mitochondrial proteomics with sirna knockdown to reveal acat1 and mdh2 in the development of *-resistant uterine cancer
Application : WB
Reactivity : Human
May EW et al. Pharmacol Res 2014; (Epub) identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
Li JM et al. Environ Mol Mutagen 2013; 54 (6) : 429-49 Hyaluronic acid-dependent protection against UVB-damaged human corneal cells.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169