Wild-type (WT) and MDH2 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody (GTX105870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody (GTX105870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with MDH2 antibody (GTX105870) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MDH2 antibody detects MDH2 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
MDH2 antibody (GTX105870) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:1000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

MDH2 antibody detects MDH2 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
MDH2 antibody (GTX105870) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MDH2 antibody detects MDH2 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
MDH2 antibody (GTX105870) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:1000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

MDH2 antibody detects MDH2 protein by western blot analysis.
A. 50 μg rat lung lysate/extract
10% SDS-PAGE
MDH2 antibody (GTX105870) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MDH2 antibody detects MDH2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Sample (20 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX105870 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.