United States (US)

MDM2 antibody [HDM2-323]

Cat No. GTX10567

Host Mouse
Clonality Monoclonal
Clone Name HDM2-323
Isotype IgG2a
Application WB, ELISA
Reactivity Human, Mouse

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )




0.01M PBS pH7.4, 15 mM sodium azide


Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.


~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species



synthetic peptide corresponding to amino acids 389-402 of human MDM2 conjugated to KLH.


Purified immunoglobulin




For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


Mdm2 Proto-Oncogene,Actfs,Hdmx,Hdm2,Mdm2


MDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90 kD that forms a complex with the p53 tumor suppressor protein. Human MDM2 was identified as a homologous product of the 'murine double minute 2' gene (mdm2). The MDM2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. Forming a tight complex with the p53 gene, the MDM2 oncogene can inhibit p53-mediated transactivation, MDM2 also binds to p53 protein. Inactivation of tumor suppressor genes leads to deregulated cell proliferation and is a key factor in human tumorigenesis. p53 can be subjected to negative regulation by the product of a single cellular protooncogene. The interference of binding to p53 prevents the interaction of MDM2 and its regulation of the transcriptional activity of p53 in vivo. Direct association of p53 with the cellular protein MDM2 results in ubiquitination and subsequent degradation of p53. MDM2-p53 complexes were preferentially found in S/G2M phases of the cell cycle. The MDM2 gene is alternatively spliced, producing 5 additional splice variant transcripts from the full length MDM2 gene. The alternatively spliced transcripts tend to be expressed in tumorigenic tissue, whereas the full length MDM2 transcript is expressed in normal tissue.


Research Area

Package List Price ($)
$ 329