GeneTex
United States (US)

MIP3 beta antibody

Cat No. GTX10389

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, Neut
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2 μg/ml
ICC/IF 5-15 μg/ml
Neut 0.8-4 μg/ml
Not tested in other applications.

Calculated MW

12 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS containing carbohydrates

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Recombinant mouse MIP-3β, expressed in Escherichia coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Chemokine (C-C Motif) Ligand 19,Ckb11,Elc,Mip3B,Scya19,Exodus-3,Ccl19

Background

MIP3 chemokines are among many novel beta chemokines recently identified through bioinformatics in the Expressed Sequence Tag (EST) database. They are distantly related to other beta chemokines (20-30% aa sequence homology). MIP3 chemokines are useful probes for studying the biology of inflammation and cell migration. Their expression is strongly up-regulated by inflammatory signals and down-regulated by the antiinflammatory cytokine IL10. MIP3 genes do not share chromosomal locations with other beta chemokines that are mainly clustered on chromosome 17. MIP3 alpha is also known as LARC (liver and activation regulated chemokine) and as exodus. It is expressed predominately in lymph nodes, appendix, PBL, fetal liver, fetal lung and some cell lines. Synthetic or recombinant MIP3 alpha has been shown to be chemotactic for cultured human lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. MIP3 alpha binds to the chemokine receptor CCR6. The MIP3 alpha gene has been mapped to chromosome 2. MIP3 beta is also known as ELC (EBI1-1 ligand chemokine) and is constitutively expressed in various lymphoid tissues (thymus, lymph nodes, appendix and spleen). MIP3 beta is a chemoattractant for cultured human lymphcytes, dendritic cells, human Tlymphoblastoid CEM NKR2 and hemopoietic progenitor cells. It may be involved in G Protein activation in human NK cells. MIP3 beta is a unique functional ligand for CCR7. The MIP3 beta gene has been mapped to chormosome 9p13.

Database

Package List Price ($)
$ 319