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MLKL (phospho Ser358) antibody

Orthogonal
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in Western Blot (WB). GTX00973
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in ELISA (ELISA). GTX00973

Cat No. GTX00973

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($339)
See all MLKL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human MLKL around the phosphorylation site of Ser358.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MLKL , hMLKL , mixed lineage kinase domain like , mixed lineage kinase domain like pseudokinase

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of mouse muscle tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of human gastric cancer tissue sections using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in Western Blot (WB). GTX00973

GTX00973 WB Image

Orthogonal

WB analysis of HeLa cell lysate using GTX00973 MLKL (phospho Ser358) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of rat heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of rat heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of rat muscle tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in ELISA (ELISA). GTX00973

GTX00973 ELISA Image

Orthogonal

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00973 MLKL (phospho Ser358) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339