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MLKL (phospho Ser358) antibody

Orthogonal
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in ELISA (ELISA). GTX00973
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973
Anti-MLKL (phospho Ser358) antibody used in Western Blot (WB). GTX00973
Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

Cat. No. GTX00973

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all MLKL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human MLKL around the phosphorylation site of Ser358.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MLKL , hMLKL , mixed lineage kinase domain like , mixed lineage kinase domain like pseudokinase

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of rat muscle tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of mouse muscle tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in ELISA (ELISA). GTX00973

GTX00973 ELISA Image

Orthogonal

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00973 MLKL (phospho Ser358) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of rat heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of human gastric cancer tissue sections using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MLKL (phospho Ser358) antibody used in Western Blot (WB). GTX00973

GTX00973 WB Image

Orthogonal

WB analysis of HeLa cell lysate using GTX00973 MLKL (phospho Ser358) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

Anti-MLKL (phospho Ser358) antibody used in IHC (Paraffin sections) (IHC-P). GTX00973

GTX00973 IHC-P Image

IHC-P analysis of rat heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

REFERENCE

Application Reference

Y Ma et al. J Oral Rehabil 2023; Low-frequency pulsed electromagnetic fields alleviate the condylar cartilage degeneration and synovitis at the early stage of temporomandibular joint osteoarthritis
Application : WB
Parul Katiyar et al. Curr Res Toxicol 2022; 3 : 100072 Triclosan-induced neuroinflammation develops caspase-independent and TNF-α signaling pathway associated necroptosis in Neuro-2a cells.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419