MLKL (phospho Ser358) antibody

Cat. No. GTX00973

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($419)

See all MLKL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:2000
IHC-P	1:50-1:200
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human MLKL around the phosphorylation site of Ser358.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MLKL , hMLKL , mixed lineage kinase domain like , mixed lineage kinase domain like pseudokinase

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Database

Gene ID: 197259 MLKL
UniProt: Q8NB16 MLKL

Research Area

DATA IMAGES

GTX00973 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX00973 IHC-P Image

IHC-P analysis of rat muscle tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX00973 IHC-P Image

IHC-P analysis of mouse muscle tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX00973 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00973 MLKL (phospho Ser358) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

GTX00973 IHC-P Image

IHC-P analysis of rat heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX00973 IHC-P Image

IHC-P analysis of human gastric cancer tissue sections using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX00973 WB Image

WB analysis of HeLa cell lysate using GTX00973 MLKL (phospho Ser358) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

GTX00973 IHC-P Image

REFERENCE

Application Reference

Parul Katiyar et al. Curr Res Toxicol 2022; 3 : 100072 Triclosan-induced neuroinflammation develops caspase-independent and TNF-α signaling pathway associated necroptosis in Neuro-2a cells.

Application : WB

Reactivity : Mouse

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REVIEW

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Application Reference

Parul Katiyar et al. Curr Res Toxicol 2022; 3:100072 Triclosan-induced neuroinflammation develops caspase-independent and TNF-α signaling pathway associated necroptosis in Neuro-2a cells.

Application : WB

Reactivity : Mouse

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 419

Qty

MLKL (phospho Ser358) antibody

Cat. No. GTX00973

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX00973 IHC-P Image

GTX00973 IHC-P Image

GTX00973 IHC-P Image

GTX00973 IHC-P Image

GTX00973 ELISA Image

GTX00973 IHC-P Image

GTX00973 IHC-P Image

GTX00973 WB Image

GTX00973 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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