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MUC1 antibody [M8C9]

Cat No. GTX10123

Host Mouse
Clonality Monoclonal
Clone Name M8C9
Isotype IgG
Application ELISA, sELISA
Reactivity Human
Package
100 μg ($239)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ELISA Assay dependent
sELISA Assay dependent

Note :

ELISA
This antibody can be used for detection of unglycosylated and glycosylated tumor-associated MUC1 mucins in human serum.

sELISA
Capture : GTX10123, Dection: GTX10123 (detection of unglycosylated MUC1) or Capture : GTX10123, Dection: GTX10118/GTX28323 (detection of glycosylated MUC1)

Not tested in other applications.

Calculated MW

25.41 kDa. ( Note )

Specificity/Sensitivity

Clone CM1 , M9E7, M8C9 bind with high efficiency with different regions of GVTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule. These antibodies react with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1 prepared from tumor fluids (T47D cells) or as a result of chemical treatment (NaJO4) of human milk MUC1. They do not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific antibodies and belong to monoclonal antibody cluster 1-4 according to ISOMB TD-4 classification. No cross-reactivity with egg white avidin.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.09 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant (E. coli) VNTR20 (tandem repeat in MUC1 core) polypeptide.This antibody binds efficiently with different regions of GVTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mucin 1, Cell Surface Associated , Admckd , Admckd1 , Ca 15-3 , Cd227 , Ema , H23Ag , Kl-6 , Mam6 , Mcd , Mckd , Mckd1 , Muc-1 , Muc-1/Sec , Muc-1/X , Muc1/Zd , Pem , Pemt , Pum , Muc1

Background

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

Database

Research Area

REFERENCE
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REVIEW
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Package List Price ($)
$ 239