Application Note
Recommended Starting Dilutions:
For ELISA: Use at a dilution of 1:10,000.
For IHC-P: Use at a dilution of 1:200.
For IP: Use at a dilution of 1:200.
Optimal dilutions should be determined experimentally by the researcher.
Calculated MW
Specificity/Sensitivity
The antibody will detect deglycosylated MUCIN1 protein.
Form
Liquid
Buffer
Tris, HCl /glycine buffer pH 7.4-7.8; 30% glycerol and 0.5% BSA along with cryo-protective agents; 0.05% sodium azide as preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide (60 aa long) representing a 20 amino acid repeat sequence from mucin 1.
Purification
Immunogen affinity purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
Mucin 1, Cell Surface Associated,Admckd,Admckd1,Ca 15-3,Cd227,Ema,H23Ag,Kl-6,Mam6,Mcd,Mckd,Mckd1,Muc-1,Muc-1/Sec,Muc-1/X,Muc1/Zd,Pem,Pemt,Pum,Muc1
Cellular Localization
Cell Membrane and Cytoplasmic
Background
MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.
Database
Research Area
