GeneTex
United States (US)

MUC1 antibody

GTX14690_20160321_IHC-P_1_18121410_466.jpg
GTX14690_20160321_IHC-P_2_18121410_401.jpg

Cat No. GTX14690

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IHC-P, IP, ELISA
Reactivity Human, Monkey
APPLICATION

Application Note

Recommended Starting Dilutions: For ELISA: Use at a dilution of 1:10,000. For IHC-P: Use at a dilution of 1:200. For IP: Use at a dilution of 1:200. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

122 kDa. ( Note )

Specificity/Sensitivity

The antibody will detect deglycosylated MUCIN1 protein.
PROPERTIES

Form

Liquid

Buffer

Tris, HCl /glycine buffer pH 7.4-7.8; 30% glycerol and 0.5% BSA along with cryo-protective agents; 0.05% sodium azide as preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide (60 aa long) representing a 20 amino acid repeat sequence from mucin 1.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mucin 1, Cell Surface Associated,Admckd,Admckd1,Ca 15-3,Cd227,Ema,H23Ag,Kl-6,Mam6,Mcd,Mckd,Mckd1,Muc-1,Muc-1/Sec,Muc-1/X,Muc1/Zd,Pem,Pemt,Pum,Muc1

Cellular Localization

Cell Membrane and Cytoplasmic

Background

MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.

Database

Research Area

DATA IMAGES
GTX14690_20160321_IHC-P_1_18121410_466.jpg

GTX14690 IHC-P Image

Baboon Lung- MUC1. Primary Antibody at 1:100 dilution in IHC Blocking Buffer, DAB (brown) staining and Hematoxylin QS (blue) counterstain, 40x magnification on Leica DM4000 microscope. FFPE sertion.

GTX14690_20160321_IHC-P_2_18121410_401.jpg

GTX14690 IHC-P Image

Baboon Lung- MUC1. Primary Antibody at 1:100 dilution in IHC Blocking Buffer, DAB (brown) staining and Hematoxylin QS (blue) counterstain, 40x magnification on Leica DM4000 microscope. FFPE sertion.