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MURF1 antibody

Anti-MURF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02420
Anti-MURF1 antibody used in Western Blot (WB). GTX02420

Cat No. GTX02420

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:1500-1:50000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to the N-terminus of the Human TRIM63

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tripartite motif containing 63 , IRF , MURF1 , MURF2 , RNF28 , SMRZ

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-MURF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02420

GTX02420 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX02420 MURF1 antibody.
Red : Primary antibody
Dilution : 1:5000
Permeabilzation : 0.3% Triton X-100

Anti-MURF1 antibody used in Western Blot (WB). GTX02420

GTX02420 WB Image

WB analysis of NIH-3T3 whole cell lysate (Lane A) using GTX02420 MURF1 antibody.
Dilution : 1:500
Loading : 30 μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 339