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MURF1 antibody

Orthogonal
Anti-MURF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX24125
Anti-MURF1 antibody used in Immunohistochemistry (IHC). GTX24125

Cat. No. GTX24125

Reference 3

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Rat, Zebrafish
Package
50 μg ($399)
See all MURF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100 - 1:2000
IHC-P 1.25μg/ml
IHC-Fr Assay dependent
IHC 1:10 - 1:500

Note :

IHC-P
Human Skeletal Muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX24125-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence DYKSSLIQDGNPM-C, from the N Terminus of the protein sequence according to NP_115977.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_370559

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 63 , IRF , MURF1 , MURF2 , RNF28 , SMRZ

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-MURF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX24125

GTX24125 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX24125 MURF1 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 1.25μg/ml

Anti-MURF1 antibody used in Immunohistochemistry (IHC). GTX24125

GTX24125 IHC Image

Orthogonal

The data was published in the journal PLoS One in 2012. PMID: 22235261

REFERENCE

Application Reference

Cunha JE et al. Sci Rep 2019; 9 (1) : 6366 Knee osteoarthritis induces atrophy and neuromuscular junction remodeling in the quadriceps and tibialis anterior muscles of rats.
Application : WB
Reactivity : Rat
Rowan SL et al. PLoS One 2012; 7 (1) : e29082 Denervation causes fiber atrophy and myosin heavy chain co-expression in senescent skeletal muscle.
Application : IHC
Reactivity : Rat
Steffen LS et al. Dev Biol. 2007; 309 (2) : 180-92 The zebrafish runzel muscular dystrophy is linked to the titin gene.
Application : IHC-Fr
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399