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MURF1 antibody

Anti-MURF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX24125

Cat No. GTX24125

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr, IHC

Reactivity

Human, Rat, Zebrafish
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100 - 1:2000
IHC-P 1.25μg/ml
IHC-Fr Assay dependent
IHC 1:10 - 1:500

Note :

IHC-P
Human Skeletal Muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX24125-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence DYKSSLIQDGNPM-C, from the N Terminus of the protein sequence according to NP_115977.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_370559

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tripartite motif containing 63 , IRF , MURF1 , MURF2 , RNF28 , SMRZ

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-MURF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX24125

GTX24125 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX24125 MURF1 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 1.25μg/ml

REFERENCE

REVIEW

MURF1 antibody Cat No. GTX24125

Rating ( Average 5 based on 1 users reviews)
Application
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2012.PMID: 22235261
SDS
Sodium Azide.pdf
Package List Price ($)
$ 319