Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [N2C2], Internal (GTX110256) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using MX1(GTX110256) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MX1 antibody [N2C2], Internal detects MX1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human MX1-transfected 293T cells.
7.5% SDS-PAGE
MX1 antibody [N2C2], Internal (GTX110256) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MX1 antibody [N2C2], Internal detects MX1 protein at cytoplasm in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
MX1 antibody [N2C2], Internal (GTX110256) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MX1 antibody [N2C2], Internal detects MX1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MX1 protein stained by MX1 antibody [N2C2], Internal (GTX110256) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

The data was published in the journal PLoS One in 2016. PMID: 27227879

The data was published in the journal PLoS One in 2012.PMID: 23139774