For FACS, IA, IHC-Fr and WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.
Reacts specificly with human 180 kDa mannose receptor
Phosphate-buffered saline containing 0.1% BSA and 0.02% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Mannose Receptor C-Type 1,Cd206,Clec13D,Clec13Dl,Mmr,Mrc1L1,Ba541I19.1,Hmr,Mrc1
The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognizes a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MRs are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines such as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases at 37ºC with prolonged incubation time probably due to internalization of the MR-antibody-complex. The antibody prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.