Human tissue extract (10 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Mitochondrial Ferritin antibody [HL3521] (GTX641418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Mitochondrial Ferritin antibody [HL3521] (GTX641418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with Mitochondrial Ferritin antibody [HL3521] (GTX641418) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Mitochondrial Ferritin antibody [HL3521] (GTX641418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.