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Mouse IL18 protein (active)

Mouse IL18 protein (active). GTX00284-pro
Mouse IL18 protein (active). GTX00284-pro
Mouse IL18 protein (active). GTX00284-pro

Cat No. GTX00284-pro

Application Functional Assay
Species Mouse
Package
10 μg ($259)
APPLICATION

Application Note

IL18 (interleukin 18) is a cytokine that shares similarities in structure with IL1, and is produced by macrophages and other cells. IL18 has been reported to stimulate T cells proliferation and NK cell activity, besides, murine IL18 has been found to enchance the production of INFγ in spleen cells in vitro. Thus, a stimulation assay was conducted to detect the activity of IL18 using murine spleen cells. Briefly, murine spleen cells were seeded into wells of 6-well plates at a density of 2×1x10⁶ cells/ml in RPMI-1640 with the addition of various concentrations of IL18. After incubation for 48 hours, the concentration of INFγ in the cell supernatant was detected using an ELISA kit. INFγ levels in the cell supernatant of spleen cells increased significantly after stimulated with IL18.

Observed MW

19 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asn36~Ser192 (NP_032386.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 18,Igif,Il-18

Database

Research Area

DATA IMAGES
Mouse IL18 protein (active). GTX00284-pro

GTX00284-pro Functional Assay Image

ELISA detection of secreted IFN gamma protein activation from murine spleen cells induced by GTX00284-pro Mouse IL18 protein (active) for 48hrs.
Recombinant protein concentrations: 1, 10, 100, and 1000 ng/ml.

Mouse IL18 protein (active). GTX00284-pro

GTX00284-pro Image

WB analysis of GTX00284-pro Mouse IL18 protein (active).

Mouse IL18 protein (active). GTX00284-pro

GTX00284-pro Image

SDS-PAGE analysis of GTX00284-pro Mouse IL18 protein (active).

REFERENCE
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