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Mouse LIF protein (active)

Mouse LIF protein (active). GTX00320-pro
Mouse LIF protein (active). GTX00320-pro
Mouse LIF protein (active). GTX00320-pro

Cat No. GTX00320-pro

Application Functional Assay
Species Mouse
Package
10 μg ($269)
APPLICATION

Application Note

Leukemia inhibitory factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. LIF as a cytokine also has another fuction including: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. Besides, Colony Stimulating Factor Receptor, Granulocyte (GCSFR) has been identified as an interactor of LIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse LIF and recombinant mouse GCSFR. Briefly, LIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to GCSFR-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-LIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of LIF and GCSFR was in a dose dependent manner.

Observed MW

21 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro25~Phe203 (NP_001034626.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

leukemia inhibitory factor

Database

Research Area

DATA IMAGES
Mouse LIF protein (active). GTX00320-pro

GTX00320-pro Functional Assay Image

Functional ELISA analysis of GTX00320-pro Mouse LIF protein (active) which can bind immobilized GCSFR protein.

Mouse LIF protein (active). GTX00320-pro

GTX00320-pro Image

SDS-PAGE analysis of GTX00320-pro Mouse LIF protein (active).

Mouse LIF protein (active). GTX00320-pro

GTX00320-pro Image

WB analysis of GTX00320-pro Mouse LIF protein (active).

REFERENCE
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