GeneTex
  • Country / Location Selection

United States (US)

Mouse MMP2 protein (active)

Mouse MMP2 protein (active). GTX00073-pro
Mouse MMP2 protein (active). GTX00073-pro
Mouse MMP2 protein (active). GTX00073-pro

Cat No. GTX00073-pro

Application Functional Assay
Species Mouse
Package
10 μg ($279)
APPLICATION

Application Note

MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 72kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of MMP2 were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate–polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1 mg/ml) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In the experiment, using a heat-denatured MMP2 protein was as a negative control, and blood sample was as a positive control.

Observed MW

63 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Tyr110~Cys660 (NP_032636.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

matrix metallopeptidase 2 , Clg4a , GelA , MMP-2

Background

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that hydrolyzes collagens, gelatins, laminin, fibronectin and elastin. Mice lacking the encoded protein exhibit suppressed angiogenesis and attenuated features of human multicentric osteolysis with arthritis including abnormal skeletal and craniofacial development. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES
Mouse MMP2 protein (active). GTX00073-pro

GTX00073-pro Functional Assay Image

Gelatin hydrolysis by various concentrations of GTX00073-pro Mouse MMP2 protein (active).
Positive control: Blood sample
Negative control: Heat-denatured MMP2 protein

Mouse MMP2 protein (active). GTX00073-pro

GTX00073-pro Image

WB analysis of GTX00073-pro Mouse MMP2 protein (active).

Mouse MMP2 protein (active). GTX00073-pro

GTX00073-pro Image

SDS-PAGE analysis of GTX00073-pro Mouse MMP2 protein (active).

REFERENCE
There are currently no references for Mouse MMP2 protein (active) (GTX00073-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Mouse MMP2 protein (active) (GTX00073-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 279