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Mouse SFTPC protein (active)

Mouse SFTPC protein (active). GTX00326-pro
Mouse SFTPC protein (active). GTX00326-pro
Mouse SFTPC protein (active). GTX00326-pro

Cat No. GTX00326-pro

Application Functional Assay
Species Mouse
Package
10 μg ($309)
APPLICATION

Application Note

Surfactant associated proteins (SPC), is one of the pulmonary surfactant proteins. It is a membrane protein which manufactures surfactant. The propeptide of pulmonary surfactant C has an N-terminal alpha-helical segment whose suggested function was stabilization of the protein structure, since the latter can irreversibly transform from its native alpha-helical structure to beta-sheet aggregates and form amyloid fibrils. Besides, Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) has been identified as an interactor of SPC, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SPC and recombinant mouse EIF2aK3. Briefly, SPC were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to EIF2aK3-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-SPC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of SPC and EIF2aK3 was in a dose dependent manner.

Observed MW

14 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Phe94~Ile193

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

surfactant associated protein C,Bricd6,SP-C,SP5,SPC,Sftp-2,Sftp2,pro-SpC

Database

Research Area

DATA IMAGES
Mouse SFTPC protein (active). GTX00326-pro

GTX00326-pro Functional Assay Image

Functional ELISA analysis of GTX00326-pro Mouse SFTPC protein (active) which can bind immobilized EIF2aK3 protein.

Mouse SFTPC protein (active). GTX00326-pro

GTX00326-pro Image

WB analysis of GTX00326-pro Mouse SFTPC protein (active).

Mouse SFTPC protein (active). GTX00326-pro

GTX00326-pro Image

SDS-PAGE analysis of GTX00326-pro Mouse SFTPC protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 309