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Mouse alpha Synuclein protein (active, Pre-Formed Fibrils)

Mouse alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17671-pro
Mouse alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17671-pro
Mouse alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17671-pro
Mouse alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17671-pro

Cat. No. GTX17671-pro

Application

 Functional Assay

Species

 Mouse
Package
200 μg ($689),
100 μg ($439)
See all alpha Synuclein protein products
PRODUCT

Summary

Active Mouse Recombinant Alpha Synuclein Pre-formed Fibrils (Type 1)

APPLICATION

Application Note

Endogenous alpha-synuclein phosphorylation. 100 μM alpha synuclein protein monomer (GTX17670-pro) seeded with 10 nM alpha synuclein protein PFF (GTX17671-pro) in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated an increased fluorescence intensity after incubation at 37ºC with shaking at 600 rpm for 24 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
*For best results, sonicate immediately prior to use.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full-length without tagged; MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKGEEGYPQE GILEDMPVDP GSEAYEMPSE EGYQDYEPEA

Expression System

E. coli

Purification

Purified by ion-exchange chromatography

Purity

> 95% by SDS-PAGE

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synuclein, alpha , synuclein, alpha , NACP , alpha-Syn , alphaSYN

Database

Research Area

DATA IMAGES

Mouse alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17671-pro

GTX17671-pro Functional Assay Image

Primary rat hippocampal neurons (DIV16) show lewy body inclusion formation and loss of cells when treated with active mouse Alpha Synuclein Protein Preformed Fibrils (GTX17671-pro) at 4 μg/ml (D-F) on DVI2, but not when treated with a control (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 3% formaldehyde from PFA for 20 min. Blocker: 1:1 PBS:LiCOR Odyssey Block (LiCOR, 927-40010) and 30 mL/mL of 0.1% triton-X 100 for 30 min. Primary Antibody: Mouse anti-pSer129 Antibody (1:1000) and Rabbit anti-pSer129 (1:800) for 24 hours at 4ºC. Secondary Antibody: ATTO 546 Donkey Anti-Mouse (1:700) and ATTO 488 Donkey Anti-Rabbit (1:700) for 1 hour at RT (composite green). Counterstain: Hoechst (blue) nuclear stain at 1:3000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.

Mouse alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17671-pro

GTX17671-pro Functional Assay Image

Immunohistochemistry analysis of rat brain injected with active mouse alpha synuclein PFFs (GTX17671-pro). Species: Female Sprague-Dawley Rat. Rat was injected with 2μL active mouse alpha synuclein PFFs (GTX17671-pro) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.

Mouse alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17671-pro

GTX17671-pro Image

Active alpha synuclein preformed fibrils (GTX17671-pro) seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers (GTX17670-pro). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of active alpha synuclein preformed fibrils (GTX17671-pro) is combined with 100 μM of alpha synuclein monomer (GTX17670-pro), as compared to active alpha synuclein preformed fibrils (GTX17671-pro) or alpha Synuclein monomer (GTX17670-pro) alone. Thioflavin T ex = 450 nm, em = 485 nm.

Mouse alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17671-pro

GTX17671-pro Image

SDS-PAGE of ~14 kDa active Mouse alpha Synuclein protein (active, fibrils) (GTX17671-pro).
Lane 1: Molecular Weight Ladder (MW). Lane 2: active Mouse alpha Synuclein protein (active, fibrils) (2 μg) (GTX17671-pro).

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SDS
PBS.pdf
Package List Price ($)
$ 689
$ 439