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Mouse iNOS protein (active)

Mouse iNOS protein (active). GTX00325-pro
Mouse iNOS protein (active). GTX00325-pro
Mouse iNOS protein (active). GTX00325-pro

Cat No. GTX00325-pro

Application Functional Assay
Species Mouse
Package
10 μg ($319)
APPLICATION

Application Note

Nitric oxide synthase 2, inducible (NOS2) is a member of Nitric oxide synthases (NOSs) family. Nitric oxide synthases (NOSs) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule. It helps modulate vascular tone, insulin secretion, airway tone, and peristalsis, and is involved in angiogenesis and neural development. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of NOS2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse NOS2 and recombinant mouse UCHL5. Briefly, NOS2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-NOS2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of NOS2 and UCHL5 was in a dose dependent manner.

Observed MW

20 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asp43~Thr213 (NP_001300850.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nitric oxide synthase 2, inducible,MAC-NOS,NOS-II,Nos-2,Nos2a,i-NOS,iNOS

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES
Mouse iNOS protein (active). GTX00325-pro

GTX00325-pro Functional Assay Image

Functional ELISA analysis of GTX00325-pro Mouse iNOS protein (active) which can bind immobilized UCHL5 protein.

Mouse iNOS protein (active). GTX00325-pro

GTX00325-pro Image

WB analysis of GTX00325-pro Mouse iNOS protein (active).

Mouse iNOS protein (active). GTX00325-pro

GTX00325-pro Image

SDS-PAGE analysis of GTX00325-pro Mouse iNOS protein (active).

REFERENCE
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