Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [GT2377] (GTX634485) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [GT2377] (GTX634485) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.