Western Blot analysis (reducing conditions) of recombinant protein EsaT-6 using polyclonal anti-EsaT-6 antibody (GTX13960).
Lane 1, 3, 5: cell lysates of non-transfected E. coli (negative controls)
Lane 2, 4, 6: cell lysate of EsaT-6-transfected E. coli; dilution (2) 1 μg/ml, (4) 0.2 μg/ml,(6) 0.1 μg/ml; detection with Donkey anti-rabbit / HRP secondary antibody