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NCAM antibody [SPM128]

Anti-NCAM antibody [SPM128] used in Flow cytometry (FACS). GTX34891
Anti-NCAM antibody [SPM128] used in IHC (Paraffin sections) (IHC-P). GTX34891

Cat. No. GTX34891

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM128

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat, Zebrafish
Package
100 μg ($529)
See all NCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Product Note

This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Membrane preparation of a small cell lung carcinoma

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD56 , MSK39 , NCAM , NCAM1 , neural cell adhesion molecule 1

Cellular Localization

Cell membrane,Secreted

Background

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-NCAM antibody [SPM128] used in Flow cytometry (FACS). GTX34891

GTX34891 FACS Image

FACS analysis of human monocytes using GTX34891 NCAM antibody [SPM128].

Anti-NCAM antibody [SPM128] used in IHC (Paraffin sections) (IHC-P). GTX34891

GTX34891 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX34891 NCAM antibody [SPM128].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529