*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
It recognizes a protein of 43kDa, which is identified as N-myc downstream-regulated gene 1 protein (NDRG1). The NDRG) family is comprised of four members, NDRG1, NDRG2, NDRG3 and NDRG4, which share 57-65% homology. The NDRG1 gene is ubiquitously expressed, but it is expressed most prominently in placental membranes and prostate, kidney, small intestine and ovary tissue. NDRG1 is a direct transcriptional target gene of p53 to mediated cell death and apoptosis. NDRG1 gene expression is induced by several compounds, including nickel, and produces a protein involved in stress responses, hormone responses, cell growth and differentiation. The reduced expression of NDRG1 has been found to be associated with tumor metastasis in a variety of tumors, including cancers of the breast, colon, prostate, oral cavity and oropharynx. Reportedly, overexpression of NDRG1 in hepatocellular carcinoma is an indicator of tumor aggressiveness.
Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Recombinant fragment of human NDRG1 fragment (aa162-283) (exact sequence is proprietary)
Ab purified from Bioreactor Concentrate by Protein A/G
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
N-Myc Downstream Regulated 1,Cap43,Cmt4D,Drg-1,Drg1,Gc4,Hmsnl,Ndr1,Nmsl,Proxy1,Rit42,Rtp,Targ1,Tdd5,Ndrg1
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
GTX34896 WB Image
Western Blot of Kidney and HeLa Cell Lysate using NDRG1 Monoclonal Antibody (NDRG1/1383)