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NDRG1 antibody [NDRG1/1383]

Anti-NDRG1 antibody [NDRG1/1383] used in Western Blot (WB). GTX34896

Cat No. GTX34896

Host Mouse
Clonality Monoclonal
Clone Name NDRG1/1383
Isotype IgG3
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0ug/ml
ICC/IF 0.5-1ug/ml
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Specificity/Sensitivity

It recognizes a protein of 43kDa, which is identified as N-myc downstream-regulated gene 1 protein (NDRG1). The NDRG) family is comprised of four members, NDRG1, NDRG2, NDRG3 and NDRG4, which share 57-65% homology. The NDRG1 gene is ubiquitously expressed, but it is expressed most prominently in placental membranes and prostate, kidney, small intestine and ovary tissue. NDRG1 is a direct transcriptional target gene of p53 to mediated cell death and apoptosis. NDRG1 gene expression is induced by several compounds, including nickel, and produces a protein involved in stress responses, hormone responses, cell growth and differentiation. The reduced expression of NDRG1 has been found to be associated with tumor metastasis in a variety of tumors, including cancers of the breast, colon, prostate, oral cavity and oropharynx. Reportedly, overexpression of NDRG1 in hepatocellular carcinoma is an indicator of tumor aggressiveness.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fragment of human NDRG1 fragment (aa162-283) (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

N-Myc Downstream Regulated 1,Cap43,Cmt4D,Drg-1,Drg1,Gc4,Hmsnl,Ndr1,Nmsl,Proxy1,Rit42,Rtp,Targ1,Tdd5,Ndrg1

Background

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES
Anti-NDRG1 antibody [NDRG1/1383] used in Western Blot (WB). GTX34896

GTX34896 WB Image

Western Blot of Kidney and HeLa Cell Lysate using NDRG1 Monoclonal Antibody (NDRG1/1383)