Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human NF-H. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888431
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
neurofilament heavy , CMT2CC , NFH
Background
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq]
NF-H antibody [GT114] detects NF-H protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: NF-H protein stained by NF-H antibody [GT114] (GTX634289) diluted at 1:250. Red: NeuN, stained by NeuN antibody (GTX133127) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GTX634289 IHC-Fr Image
NF-H antibody [GT114] detects NF-H protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: NF-H protein stained by NF-H antibody [GT114] (GTX634289) diluted at 1:250. Red: NeuN, stained by NeuN antibody (GTX133127) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GTX634289 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NF-H antibody [GT114] (GTX634289) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634289 IHC-P Image
NF-H antibody [GT114] detects NF-H protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NF-H antibody [GT114] (GTX634289) diluted at 1:500. Scale bar = 1 μm.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634289 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NF-H antibody [GT114] (GTX634289) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634289 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NF-H antibody (GTX634289) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634289 IHC-P Image
NF-H antibody [GT114] detects NF-H protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NF-H antibody [GT114] (GTX634289) diluted at 1:500. Scale bar = 1 μm.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Show More +
GTX634289 ICC/IF Image
NF-H antibody [GT114] detects NF-H protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NF-H protein stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).