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NF-H antibody [GT114]

Anti-NF-H antibody [GT114] used in IHC (Frozen sections) (IHC-Fr). GTX634289
Anti-NF-H antibody [GT114] used in IHC (Frozen sections) (IHC-Fr). GTX634289
Anti-NF-H antibody [GT114] used in Western Blot (WB). GTX634289
Anti-NF-H antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX634289
Anti-NF-H antibody [GT114] used in Western Blot (WB). GTX634289
Anti-NF-H antibody [GT114] used in Western Blot (WB). GTX634289
Anti-NF-H antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX634289
Anti-NF-H antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634289

Cat No. GTX634289

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT114

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all NF-H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

IMR32 , NT2D1 , rat brain

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NF-H. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888431

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

neurofilament heavy , CMT2CC , NFH

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NF-H antibody [GT114] used in IHC (Frozen sections) (IHC-Fr). GTX634289

GTX634289 IHC-Fr Image

NF-H antibody [GT114] detects NF-H protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NF-H protein stained by NF-H antibody [GT114] (GTX634289) diluted at 1:250.
Red: NeuN, stained by NeuN antibody (GTX133127) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-H antibody [GT114] used in IHC (Frozen sections) (IHC-Fr). GTX634289

GTX634289 IHC-Fr Image

NF-H antibody [GT114] detects NF-H protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NF-H protein stained by NF-H antibody [GT114] (GTX634289) diluted at 1:250.
Red: NeuN, stained by NeuN antibody (GTX133127) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-H antibody [GT114] used in Western Blot (WB). GTX634289

GTX634289 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NF-H antibody [GT114] (GTX634289) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NF-H antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX634289

GTX634289 IHC-P Image

NF-H antibody [GT114] detects NF-H protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Scale bar = 1 μm.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-H antibody [GT114] used in Western Blot (WB). GTX634289

GTX634289 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NF-H antibody [GT114] (GTX634289) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NF-H antibody [GT114] used in Western Blot (WB). GTX634289

GTX634289 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NF-H antibody (GTX634289) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NF-H antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX634289

GTX634289 IHC-P Image

NF-H antibody [GT114] detects NF-H protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Scale bar = 1 μm.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-H antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634289

GTX634289 ICC/IF Image

NF-H antibody [GT114] detects NF-H protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NF-H protein stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Habuchi H et al. PLoS One 2021; 16 (6) : e0252590 Bone marrow derived mast cells injected into the osteoarthritic knee joints of mice induced by sodium monoiodoacetate enhanced spontaneous pain through activation of PAR2 and action of extracellular ATP.
Application : IHC-P
Reactivity : Mouse
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169