NF-L antibody detects NF-L protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: NF-L stained by NF-L antibody (GTX134075) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

NF-L antibody detects NF-L protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
NF-L stained by NF-L antibody (GTX134075) diluted at 1:1500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NF-L antibody (GTX134075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NF-L antibody (GTX134075) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.