WB analysis of SH-SY5Y cell lysate using GTX30667 NF-M antibody. ~100kDa band is seen in addition to the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.
Lane A : SH-SY5Y cell
Lane B : SH-SY5Y treated with maitotoxin

ICC/IF analysis of mixed neuron/glia cell cultures using GTX30667 NF-M antibody (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.