IHC-P analysis of human brain tissue using GTX23446 NFAT5 antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

IP analysis of U2OS cell lysates using GTX23446 NFAT5 antibody.
IP reaction : 3μg antibody / 500μg lysate

ICC/IF analysis of NIH-3T3 cells using GTX23446 NFAT5 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

WB analysis of 25 ug of various whole cell lysates using GTX23446 NFAT5 antibody.
Dilution : 1:1000

IHC-P analysis of human skeletal muscle tissue using GTX23446 NFAT5 antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

ICC/IF analysis of HeLa cells using GTX23446 NFAT5 antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

ICC/IF analysis of HeLa cells using GTX23446 NFAT5 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of MCF-7 cells using GTX23446 NFAT5 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC