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NFAT5 antibody

Anti-NFAT5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23446
Anti-NFAT5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23446
Anti-NFAT5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23446
Anti-NFAT5 antibody used in IHC (Paraffin sections) (IHC-P). GTX23446
Anti-NFAT5 antibody used in Western Blot (WB). GTX23446
Anti-NFAT5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23446
Anti-NFAT5 antibody used in Immunoprecipitation (IP). GTX23446
Anti-NFAT5 antibody used in IHC (Paraffin sections) (IHC-P). GTX23446

Cat. No. GTX23446

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay, Gel supershift assays, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Monkey
Package
50 μg ($399)
See all NFAT5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:100-1:1000
IHC-P 1:20
IP 3 μg
ChIP assay Assay dependent
Gel supershift assays Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

166 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor of activated T cells 5 , NF-AT5 , NFATL1 , NFATZ , OREBP , TONEBP

Cellular Localization

Nucleus,Cytoplasm

Background

The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NFAT5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23446

GTX23446 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX23446 NFAT5 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-NFAT5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23446

GTX23446 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX23446 NFAT5 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-NFAT5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23446

GTX23446 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX23446 NFAT5 antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

Anti-NFAT5 antibody used in IHC (Paraffin sections) (IHC-P). GTX23446

GTX23446 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX23446 NFAT5 antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-NFAT5 antibody used in Western Blot (WB). GTX23446

GTX23446 WB Image

WB analysis of 25 ug of various whole cell lysates using GTX23446 NFAT5 antibody.
Dilution : 1:1000

Anti-NFAT5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23446

GTX23446 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX23446 NFAT5 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-NFAT5 antibody used in Immunoprecipitation (IP). GTX23446

GTX23446 IP Image

IP analysis of U2OS cell lysates using GTX23446 NFAT5 antibody.
IP reaction : 3μg antibody / 500μg lysate

Anti-NFAT5 antibody used in IHC (Paraffin sections) (IHC-P). GTX23446

GTX23446 IHC-P Image

IHC-P analysis of human brain tissue using GTX23446 NFAT5 antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

REFERENCE

Application Reference

Master A et al. Curr Eye Res 2021; : 1-10 An Improved Ocular Impression Cytology Method: Quantitative Cell Transfer to Microscope Slides Using a Novel Polymer.
Reactivity : Rabbit
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399