IHC-P analysis of human prostate tissue using GTX48667 NFYA antibody.
Dilution : 10 μg/mL

CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Load: 35 μg per lane. Primary antibody: NF-YA antibody at 1:1,000 for overnight at 4ºC. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: NF-YA recognized as a doublet of ~ 42 kDa in size. Other band(s): none.

GeneTex's anti-NF-Y (A Subunit) antibody was diluted at 10 μg/mL for 1 h at RT to detect NF-YA in human prostate tissue. Tissue was formalin fixed paraffin embedded. Antigen retrieval: not required. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. The image shows the localization of antibody as the precipitated red signal with a hematoxylin purple nuclear counterstain. Localization: NF-Y is nuclear.