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NFYB antibody [N1C3-2]

Anti-NFYB antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX114585
Anti-NFYB antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX114585
Anti-NFYB antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114585
Anti-NFYB antibody [N1C3-2] used in Western Blot (WB). GTX114585

Cat. No. GTX114585

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NFYB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624225

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear transcription factor Y subunit beta , CBF-A , CBF-B , HAP3 , NF-YB

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NFYB antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX114585

GTX114585 IHC-P Image

NFYB antibody [N1C3-2] detects NFYB protein at nucleus in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
NFYB antibody [N1C3-2] (GTX114585) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFYB antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX114585

GTX114585 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using NFYB(GTX114585) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFYB antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114585

GTX114585 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFYB(GTX114585) antibody at 1:500 dilution.

Anti-NFYB antibody [N1C3-2] used in Western Blot (WB). GTX114585

GTX114585 WB Image

Sample (20 ug )
A: HeLa
12% SDS PAGE
GTX114585 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169