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NFYB antibody [N1C3-2]

Anti-NFYB antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX114585
Anti-NFYB antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX114585
Anti-NFYB antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114585
Anti-NFYB antibody [N1C3-2] used in Western Blot (WB). GTX114585

Cat. No. GTX114585

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NFYB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624225

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear transcription factor Y subunit beta , CBF-A , CBF-B , HAP3 , NF-YB

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NFYB antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX114585

GTX114585 IHC-P Image

NFYB antibody [N1C3-2] detects NFYB protein at nucleus in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
NFYB antibody [N1C3-2] (GTX114585) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFYB antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX114585

GTX114585 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using NFYB(GTX114585) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFYB antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114585

GTX114585 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFYB(GTX114585) antibody at 1:500 dilution.

Anti-NFYB antibody [N1C3-2] used in Western Blot (WB). GTX114585

GTX114585 WB Image

Sample (20 ug )
A: HeLa
12% SDS PAGE
GTX114585 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169