GeneTex

  • Country / Location Selection

United States (US)

NFYB antibody

Orthogonal
Anti-NFYB antibody used in Western Blot (WB). GTX121922
Anti-NFYB antibody used in IHC (Paraffin sections) (IHC-P). GTX121922
Anti-NFYB antibody used in Western Blot (WB). GTX121922
Anti-NFYB antibody used in IHC (Whole mount) (IHC-Wm). GTX121922
Anti-NFYB antibody used in IHC (Paraffin sections) (IHC-P). GTX121922
Anti-NFYB antibody used in Western Blot (WB). GTX121922
Anti-NFYB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121922
Anti-NFYB antibody used in Western Blot (WB). GTX121922
Anti-NFYB antibody used in ChIP assay (ChIP assay). GTX121922
Anti-NFYB antibody used in Immunoprecipitation (IP). GTX121922
Anti-NFYB antibody used in IHC (Paraffin sections) (IHC-P). GTX121922

Cat. No. GTX121922

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all NFYB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722365

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear transcription factor Y subunit beta , CBF-A , CBF-B , HAP3 , NF-YB

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NFYB antibody used in Western Blot (WB). GTX121922

GTX121922 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFYB antibody used in IHC (Paraffin sections) (IHC-P). GTX121922

GTX121922 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using NFYB antibody (GTX121922) at 1:300 dilution.

Anti-NFYB antibody used in Western Blot (WB). GTX121922

GTX121922 WB Image

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NFYB antibody used in IHC (Whole mount) (IHC-Wm). GTX121922

GTX121922 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using NFYB antibody (GTX121922) at 1:200 dilution.

Anti-NFYB antibody used in IHC (Paraffin sections) (IHC-P). GTX121922

GTX121922 IHC-P Image

NFYB antibody detects NFYB protein at nucleus in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
NFYB antibody (GTX121922) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFYB antibody used in Western Blot (WB). GTX121922

GTX121922 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFYB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121922

GTX121922 ICC/IF Image

NFYB antibody detects NFYB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFYB protein stained by NFYB antibody (GTX121922) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

Anti-NFYB antibody used in Western Blot (WB). GTX121922

GTX121922 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFYB antibody used in ChIP assay (ChIP assay). GTX121922

GTX121922 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-NFYB antibody. The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.
ChIP experiment and primer designs are based on Nucleic Acids Res. 2006;34(21):6272-85.

Anti-NFYB antibody used in Immunoprecipitation (IP). GTX121922

GTX121922 IP Image

NFYB antibody immunoprecipitates NFYB protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 μg whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 μg of NFYB antibody (GTX121922) 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB antibody (GTX121922) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-NFYB antibody used in IHC (Paraffin sections) (IHC-P). GTX121922

GTX121922 IHC-P Image

NFYB antibody detects NFYB protein at nucleus in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
NFYB antibody (GTX121922) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Li D et al. Theranostics 2021; 11 (11) : 5585-5604 Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for NFYB antibody (GTX121922). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169