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NFYB antibody

Anti-NFYB antibody used in Western Blot (WB). GTX121922
Anti-NFYB antibody used in ChIP assay (ChIP assay). GTX121922
Anti-NFYB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121922
Anti-NFYB antibody used in Western Blot (WB). GTX121922
Anti-NFYB antibody used in Western Blot (WB). GTX121922
Anti-NFYB antibody used in Immunoprecipitation (IP). GTX121922
Anti-NFYB antibody used in IHC (Paraffin sections) (IHC-P). GTX121922
Anti-NFYB antibody used in IHC (Paraffin sections) (IHC-P). GTX121922

Cat No. GTX121922

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear extract , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722365

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear transcription factor Y subunit beta , CBF-A , CBF-B , HAP3 , NF-YB

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NFYB antibody used in Western Blot (WB). GTX121922

GTX121922 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFYB antibody used in ChIP assay (ChIP assay). GTX121922

GTX121922 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-NFYB antibody. The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.
ChIP experiment and primer designs are based on Nucleic Acids Res. 2006;34(21):6272-85.

Anti-NFYB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121922

GTX121922 ICC/IF Image

NFYB antibody detects NFYB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFYB protein stained by NFYB antibody (GTX121922) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

Anti-NFYB antibody used in Western Blot (WB). GTX121922

GTX121922 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFYB antibody used in Western Blot (WB). GTX121922

GTX121922 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFYB antibody used in Immunoprecipitation (IP). GTX121922

GTX121922 IP Image

NFYB antibody immunoprecipitates NFYB protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 μg whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 μg of NFYB antibody (GTX121922) 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB antibody (GTX121922) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-NFYB antibody used in IHC (Paraffin sections) (IHC-P). GTX121922

GTX121922 IHC-P Image

NFYB antibody detects NFYB protein at nucleus in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
NFYB antibody (GTX121922) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFYB antibody used in IHC (Paraffin sections) (IHC-P). GTX121922

GTX121922 IHC-P Image

NFYB antibody detects NFYB protein at nucleus in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
NFYB antibody (GTX121922) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169