GeneTex
United States (US)

NFkB p105/p50 (phospho Ser337) antibody

GTX32223_20190131_WB_19013102_206.jpg
GTX32223_20190131_IHC-P_19013102_845.jpg

Cat No. GTX32223

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

105 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of NFkB p105/p50. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Factor Kappa B Subunit 1,Cvid12,Ebp-1,Kbf1,Nf-Kb1,Nf-Kappa-B1,Nf-Kappab,Nfkb-P105,Nfkb-P50,Nfkappab,P105,P50,Nfkb1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES
GTX32223_20190131_WB_19013102_206.jpg

GTX32223 WB Image

WB analysis of HeLa (A), TNF-treated HEK293T (B), NIH3T3 (C), Raw264.7 (D), rat liver (E) whole cell lysates using GTX32223 NFkB p105/p50 (phospho Ser337) antibody.

GTX32223_20190131_IHC-P_19013102_845.jpg

GTX32223 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32223 NFkB p105/p50 (phospho Ser337) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Package List Price ($)
$ 319