GeneTex

  • Country / Location Selection

United States (US)

NFkB p105/p50 (phospho Ser337) antibody

Anti-NFkB p105/p50 (phospho Ser337) antibody used in IHC (Paraffin sections) (IHC-P). GTX32223
Anti-NFkB p105/p50 (phospho Ser337) antibody used in Western Blot (WB). GTX32223

Cat. No. GTX32223

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NFkB p105/p50 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of NFkB p105/p50. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887642

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor kappa B subunit 1 , CVID12 , EBP-1 , KBF1 , NF-kB1 , NF-kappa-B1 , NF-kappaB , NFKB-p105 , NFKB-p50 , NFkappaB , p105 , p50

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-NFkB p105/p50 (phospho Ser337) antibody used in IHC (Paraffin sections) (IHC-P). GTX32223

GTX32223 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32223 NFkB p105/p50 (phospho Ser337) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-NFkB p105/p50 (phospho Ser337) antibody used in Western Blot (WB). GTX32223

GTX32223 WB Image

WB analysis of HeLa (A), TNF-treated HEK293T (B), NIH3T3 (C), Raw264.7 (D), rat liver (E) whole cell lysates using GTX32223 NFkB p105/p50 (phospho Ser337) antibody.

REFERENCE

Application Reference

Taisuke Okano et al. International Journal of Endocrinology 2020; 2020 : Article ID 4139093 β-Endorphin Mediates the Development and Instability of Atherosclerotic Plaques
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for NFkB p105/p50 (phospho Ser337) antibody (GTX32223). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399