PBS with 100ug BSA 0.1% sodium azide
Keep as concentrated solution, aliquot and store at 4ºC.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Nuclear Factor Kappa B Subunit 1,Cvid12,Ebp-1,Kbf1,Nf-Kb1,Nf-Kappa-B1,Nf-Kappab,Nfkb-P105,Nfkb-P50,Nfkappab,P105,P50,Nfkb1
Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I kappa B).
NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. NF-kB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino-terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy-terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL-1alpha and TNF-alpha as mediated by IkB kinase and p38 MAPK. Serine 529 is located in a negatively charged region (amino acids 422-540) that is phosphorylated in response to phorbol myristate acetate plus calcium ionophore activation. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NF-kB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NF-kB has been associated with a number of inflammatory diseases while persistent inhibition of NF-kB leads to inappropriate immune cell development or delayed cell growth.