NFkB p65 (phospho Ser276) antibody

Cat. No. GTX04541

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all NFkB p65 products

Datasheet

View product citations for antibody GTX04541 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:2000
ICC/IF	1:100-1:500
IHC-P	1:50-1:200
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Predict Reactivity

Zebrafish, Sheep, Bovine, Dog, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human RELA(Accession Q04206), corresponding to amino acid residues around phosphorylated Ser276.

Purification

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65

Cellular Localization

Nucleus , Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Gene ID: 5970 RELA
UniProt: Q04206 RELA

Research Area

DATA IMAGES

GTX04541 IHC-P Image

IHC-P analysis of rat thymus tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

GTX04541 IHC-P Image

IHC-P analysis of human normal tissues adjacent to colorectal cancer tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

GTX04541 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

GTX04541 IHC-P Image

IHC-P analysis of human brain tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:200

GTX04541 IHC-P Image

IHC-P analysis of human normal tissues adjacent to mammary cancer tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

GTX04541 ICC/IF Image

ICC/IF analysis of PFA fixed HepG2 cells with 4μM Forskolin treatment for 30min using GTX04541 NFkB p65 (phospho Ser276) antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

GTX04541 WB Image

WB analysis of various whole cell lysates using GTX04541 NFkB p65 (phospho Ser276) antibody.
Lane 1 : MCF7 cells with serum starvation treatment follwoed by antigen-specific peptides
Lane 2 : MCF7 cells with serum starvation treatment
Lane 3 : A2780 cells with serum starvation treatment

GTX04541 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

GTX04541 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

GTX04541 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX04541 NFkB p65 (phospho Ser276) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1 μg/ml

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

Qty

NFkB p65 (phospho Ser276) antibody

Cat. No. GTX04541

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX04541 IHC-P Image

GTX04541 IHC-P Image

GTX04541 IHC-P Image

GTX04541 IHC-P Image

GTX04541 IHC-P Image

GTX04541 ICC/IF Image

GTX04541 WB Image

GTX04541 IHC-P Image

GTX04541 IHC-P Image

GTX04541 ELISA Image

REFERENCE

REVIEW

Product

Research

Support

Company

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