ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX04541 NFkB p65 (phospho Ser276) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1 μg/ml

IHC-P analysis of human colorectal cancer tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

IHC-P analysis of human mammary cancer tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

WB analysis of various whole cell lysates using GTX04541 NFkB p65 (phospho Ser276) antibody.
Lane 1 : MCF7 cells with serum starvation treatment follwoed by antigen-specific peptides
Lane 2 : MCF7 cells with serum starvation treatment
Lane 3 : A2780 cells with serum starvation treatment

ICC/IF analysis of PFA fixed HepG2 cells with 4μM Forskolin treatment for 30min using GTX04541 NFkB p65 (phospho Ser276) antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

IHC-P analysis of human normal tissues adjacent to mammary cancer tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

IHC-P analysis of human brain tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:200

IHC-P analysis of human colorectal cancer tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

IHC-P analysis of human normal tissues adjacent to colorectal cancer tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

IHC-P analysis of rat thymus tissue using GTX04541 NFkB p65 (phospho Ser276) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100