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NK1.1 antibody [PK136] (PerCP-Cy5.5)

Anti-NK1.1 antibody [PK136] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01478-11

Cat. No. GTX01478-11

Host

Mouse

Clonality

Monoclonal

Clone Name

PK136

Isotype

IgG2a

Application

FACS

Reactivity

Mouse
Package
100 μg ($279)
PRODUCT

Summary

The PK136 antibody is specific for mouse NK1.1, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. NK1.1 is prominently expressed on natural killer (NK) cells, and is correlated with NK cytotoxic effects toward virus-infected cells and tumor cells. NK1.1 is expressed on subsets of NKT cells in certain mouse strains (C57BL/6, FVB/N, and NZB), yet absent from others (AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129). Putative subsets of NK cells and their expression of NK1.1 antigen are of continuing interest, including NK1.1+/CD117+ (c-Kit) cells reported to be immunosuppressive for CD8+ T cells in a mechanism involving PD-1 and PD-L1. The PK136 antibody may be used for detection of NK1.1 expression on mouse strains including CE, B6, NZB, C58, Ma/My, ST, SJL, and FVB. The antibody is reported to react with an epitope common to NKR-P1B and NKR-P1C alloantigenic forms of NK1.1.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄ (pH7.2), 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-NK1.1 antibody [PK136] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01478-11

GTX01478-11 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01478-11 NK1.1 antibody [PK136] (PerCP-Cy5.5).
Right panel : co-stained with NK1.1 antibody [PK136] (PerCP-Cy5.5) and Mouse CD3e antibody (FITC)
Left panel : co-stained with isotype control and Mouse CD3e antibody (FITC)
antibody amount : 0.25 μg (5 μl)

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 279