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NK1.1 antibody [PK136]

Cat. No. GTX01478

Reference 9

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PK136

Isotype

 IgG2a

Application

 IHC-P, IHC-Fr, FACS, IP, Activation, Depletion, Neutralizing/Inhibition

Reactivity

 Mouse
Package
100 μg ($129)
See all NK1.1 products
PRODUCT

Summary

The PK136 antibody is specific for mouse NK1.1, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. NK1.1 is prominently expressed on natural killer (NK) cells, and is correlated with NK cytotoxic effects toward virus-infected cells and tumor cells. NK1.1 is expressed on subsets of NKT cells in certain mouse strains (C57BL/6, FVB/N, and NZB), yet absent from others (AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129). Putative subsets of NK cells and their expression of NK1.1 antigen are of continuing interest, including NK1.1+/CD117+ (c-Kit) cells reported to be immunosuppressive for CD8+ T cells in a mechanism involving PD-1 and PD-L1. The PK136 antibody may be used for detection of NK1.1 expression on mouse strains including CE, B6, NZB, C58, Ma/My, ST, SJL, and FVB. The antibody is reported to react with an epitope common to NKR-P1B and NKR-P1C alloantigenic forms of NK1.1.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Activation Assay dependent
Depletion Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄ (pH7.2), 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Purity

> 90% (determined by SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Membrane

Database

Research Area

REFERENCE

Clone Reference

2019; Combined α-programmed death-1 monoclonal antibody blockade and fractionated radiation therapy reduces tumor growth in mouse EL4 lymphoma.
Application : IHC-P
Reactivity : Mouse
2018; Phytosomal curcumin causes natural killer cell-dependent repolarization of glioblastoma (GBM) tumor-associated microglia/macrophages and elimination of GBM and GBM stem cells.
Application : Neutralizing/Inhibition
Reactivity : Mouse
2012; Lyn-dependent signaling regulates the innate immune response by controlling dendritic cell activation of NK cells.
Application : FACS, Depletion
Reactivity : Mouse
2011; Type I interferon is selectively required by dendritic cells for immune rejection of tumors.
Application : FACS, Depletion
Reactivity : Mouse
2011; The herpes simplex virus 1 IgG fc receptor blocks antibody-mediated complement activation and antibody-dependent cellular cytotoxicity in vivo.
Application : FACS, Depletion
Reactivity : Mouse
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Clone Reference
2019; Combined α-programmed death-1 monoclonal antibody blockade and fractionated radiation therapy reduces tumor growth in mouse EL4 lymphoma.
Application : IHC-P
Reactivity : Mouse
2018; Phytosomal curcumin causes natural killer cell-dependent repolarization of glioblastoma (GBM) tumor-associated microglia/macrophages and elimination of GBM and GBM stem cells.
Application : Neutralizing/Inhibition
Reactivity : Mouse
2012; Lyn-dependent signaling regulates the innate immune response by controlling dendritic cell activation of NK cells.
Application : FACS, Depletion
Reactivity : Mouse
2011; Type I interferon is selectively required by dendritic cells for immune rejection of tumors.
Application : FACS, Depletion
Reactivity : Mouse
2011; The herpes simplex virus 1 IgG fc receptor blocks antibody-mediated complement activation and antibody-dependent cellular cytotoxicity in vivo.
Application : FACS, Depletion
Reactivity : Mouse
2010; Rap1b facilitates NK cell functions via IQGAP1-mediated signalosomes.
Application : FACS, Activation
Reactivity : Mouse
2008; Sustained NKG2D engagement induces cross-tolerance of multiple distinct NK cell activation pathways.
Application : FACS, IP
Reactivity : Mouse
2005; Functional requirements for signaling through the stimulatory and inhibitory mouse NKR-P1 (CD161) NK cell receptors.
Application : IP, Neutralizing/Inhibition
Reactivity : Mouse
2001; Effects of survivin antagonists on growth of established tumors and B7-1 immunogene therapy.
Application : IHC-Fr, Depletion
Reactivity : Mouse
SDS
Sodium Azide.pdf
Package List Price ($)
$ 129