Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NKG2D antibody [N3C2], Internal (GTX104761) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NKG2D antibody [N3C2], Internal detects NKG2D protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
NKG2D antibody [N3C2], Internal (GTX104761) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

NKG2D antibody [N3C2], Internal detects NKG2D protein at cell membrane by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NKG2D stained by NKG2D antibody [N3C2], Internal (GTX104761) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.