GeneTex
United States (US)

NOTCH1 antibody

GTX28925_20160330_WB_1_18121410_414.jpg
GTX28925_20160330_WB_18121410_610.jpg
GTX28925_20160330_IHC-P_18121410_464.jpg

Cat No. GTX28925

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, ELISA, Neut
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
IHC-P 1:200
ELISA 1:20000 - 1:60000
Neut Assay dependent
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells.  Only the cleaved intracellular (activated) form (NICD) is detected.  No reactivity is detected against mouse N2, N3 or N4.  The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.1% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide: VLLSRKRRRQHGQC (aa1755-1767).This is the N-terminal sequence of the cleaved N intracellular domain (NICD).

Purification

Antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Notch 1,Aos5,Aovd1,Tan1,Hn1,Notch1

Background

Notch 1 is synthesized in the endoplasmic reticulum as an inactive form, which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

Database

Research Area

DATA IMAGES
GTX28925_20160330_WB_1_18121410_414.jpg

GTX28925 WB Image

Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot in 293 cells transiently transfected with myc-tagged mouse Notch. Bound antibody was detected with a 1:10,000 dilution of HRP conjugated Goat anti Rabbit IgG, and color development. Lane M: markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.

GTX28925_20160330_WB_18121410_610.jpg

GTX28925 WB Image

Western Blot of Rabbit anti-Notch1 antibody (GTX28925). Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17ß-estradiol. Lane 3: MCF-7 + 10 µM gamma secretase inhibitor. Load: 35 µg per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4°C. Rabbit secondary antibody at 1:10,000 for 45 min at RT.Predicted/Observed size: 80 kDa for Notch1.

GTX28925_20160330_IHC-P_18121410_464.jpg

GTX28925 IHC-P Image

IHC-P analysis of exocrine glands of human pancreas using Rabbit anti-Notch1 antibody (GTX28925) at a dilution of 1:200. Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.

REFERENCE
Package List Price ($)
$ 289