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NOTCH1 antibody

Anti-NOTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX28925
Anti-NOTCH1 antibody used in Western Blot (WB). GTX28925
Anti-NOTCH1 antibody used in Western Blot (WB). GTX28925

Cat. No. GTX28925

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NOTCH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
IHC-P 1:200
ELISA 1:20000 - 1:60000
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

Product Note

This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells.  Only the cleaved intracellular (activated) form (NICD) is detected.  No reactivity is detected against mouse N2, N3 or N4.  The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide: VLLSRKRRRQHGQC (aa1755-1767).This is the N-terminal sequence of the cleaved N intracellular domain (NICD).

Purification

Antiserum

Conjugation

Unconjugated

RRID

AB_371662

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

notch receptor 1 , AOS5 , AOVD1 , TAN1 , hN1

Cellular Localization

Cell membrane

Background

Notch 1 is synthesized in the endoplasmic reticulum as an inactive form, which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

Database

Research Area

DATA IMAGES

Anti-NOTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX28925

GTX28925 IHC-P Image

IHC-P analysis of exocrine glands of human pancreas using Rabbit anti-Notch1 antibody (GTX28925) at a dilution of 1:200. Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.

Anti-NOTCH1 antibody used in Western Blot (WB). GTX28925

GTX28925 WB Image

Western Blot of Rabbit anti-Notch1 antibody (GTX28925). Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17s-estradiol. Lane 3: MCF-7 + 10 μM gamma secretase inhibitor. Load: 35 μg per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4ºC. Rabbit secondary antibody at 1:10,000 for 45 min at RT.Predicted/Observed size: 80 kDa for Notch1.

Anti-NOTCH1 antibody used in Western Blot (WB). GTX28925

GTX28925 WB Image

Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot in 293 cells transiently transfected with myc-tagged mouse Notch. Bound antibody was detected with a 1:10,000 dilution of HRP conjugated Goat anti Rabbit IgG, and color development. Lane M: markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.

REFERENCE

Application Reference

Liu Z et al. Mol Med Rep 2021; 23 (2) : 1 MicroRNA?223?induced inhibition of the FBXW7 gene affects the proliferation and apoptosis of colorectal cancer cells via the Notch and Akt/mTOR pathways.
Application : WB
Reactivity : Human
Nwabo Kamdje AH et al. Blood Cancer J 2012; 2 (5) : e73 Role of stromal cell-mediated Notch signaling in CLL resistance to chemotherapy.
Application : Neutralizing/Inhibition
Chu PW et al. Fertil Steril 2011; 96 (5) : 1225-9 Notch 1 signaling pathway effect on implantation competency.
Application : WB
Reactivity : Mouse
Cheng X et al. Development 2008; 135 (20) : 3447-58 Numb mediates the interaction between Wnt and Notch to modulate primitive erythropoietic specification from the hemangioblast.
Application : ICC/IF
Reactivity : Mouse
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399