APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 |
| 1:25-1:250 |
| Assay dependent |
| 1:200 |
| 1:10000 |
| 1:25-1:250 |
Not tested in other applications.
Calculated MW
Product Note
The selective antibodies were generated against conserved sequences near the N terminus and the C terminal end of the protein that are unique to PGCA protein. The PGCA antibody labels a 127 to 130 kDa protein in various tissues.
PROPERTIES
Form
Liquid
Buffer
Tris/Glycine, 0.5% BSA, 30% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.85 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide corresponding to C terminal amino acids 1042-1057 of Human PGCA. Seqence: VRTYWLLGERGCSTRG
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
natriuretic peptide receptor 1 , ANPRA , ANPa , GUC2A , GUCY2A , NPRA
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).[supplied by OMIM, May 2009]
Database
Research Area
DATA IMAGES
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GTX14918 IHC Image
IHC analysis of mouse heart tissue using GTX14918 NPR-A antibody.
Red : Primary antibody
Blue : Nuclei
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GTX14918 IHC-Wm Image
IHC-Wm analysis of mouse nose tissue using GTX14918 NPR-A antibody with (Left) or without (Right) blocking peptide.
Red : Primary antibody
Green : Greneberg ganglion nerons
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GTX14918 WB Image
WB analysis of PGCA recombinant protein using GTX14918 NPR-A antibody.
Dilution : 1:500
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GTX14918 IHC Image
IHC analysis of mouse kidney tissue using GTX14918 NPR-A antibody.
Red : Primary antibody
Blue : Nuclei
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REFERENCE
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REVIEW
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