NQO1 antibody [C2C3], C-term

Cat No. GTX100235

Reference 10

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Monkey
Package	100 μl ($319), 25 μl ($169)

See all NQO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , mouse heart , rat heart

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NQO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NAD(P)H quinone dehydrogenase 1,DHQU,DIA4,DTD,NMOR1,NMORI,QR1

Cellular Localization

Cytoplasm

Background

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Gene ID: 1728 NQO1
UniProt: P15559 NQO1

Research Area

DATA IMAGES

GTX100235 IHC-P Image

NQO1 antibody [C2C3], C-term detects NQO1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NQO1 stained by NQO1 antibody [C2C3], C-term (GTX100235) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100235 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NQO1(GTX100235) antibody at 1:200 dilution.

GTX100235 WB Image

NQO1 antibody [C2C3], C-term detects NQO1 protein by western blot analysis.
A. 50 µg mouse heart lysate/extract
12% SDS-PAGE
NQO1 antibody [C2C3], C-term (GTX100235) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100235 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NQO1 antibody [C2C3], C-term (GTX100235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100235 WB Image

NQO1 antibody [C2C3], C-term detects NQO1 protein by western blot analysis.
A. 50 µg rat heart lysate/extract
12% SDS-PAGE
NQO1 antibody [C2C3], C-term (GTX100235) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100235 IHC-P Image

NQO1 antibody [C2C3], C-term detects NQO1 protein at cytoplasm in human rectal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human rectal carcinoma.
NQO1 antibody [C2C3], C-term (GTX100235) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE