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NQO1 antibody [C2C3], C-term

Anti-NQO1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100235
Anti-NQO1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100235
Anti-NQO1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100235
Anti-NQO1 antibody [C2C3], C-term used in Western Blot (WB). GTX100235
Anti-NQO1 antibody [C2C3], C-term used in Western Blot (WB). GTX100235
Anti-NQO1 antibody [C2C3], C-term used in Western Blot (WB). GTX100235

Cat. No. GTX100235

Reference 19

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399),
25 μl ($169)
See all NQO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

mouse heart , rat heart

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NQO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951017

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NAD(P)H quinone dehydrogenase 1 , DHQU , DIA4 , DTD , NMOR1 , NMORI , QR1

Cellular Localization

Cytoplasm

Background

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NQO1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100235

GTX100235 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NQO1(GTX100235) antibody at 1:200 dilution.

Anti-NQO1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100235

GTX100235 IHC-P Image

NQO1 antibody [C2C3], C-term detects NQO1 protein at cytoplasm in human rectal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human rectal carcinoma.
NQO1 antibody [C2C3], C-term (GTX100235) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NQO1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100235

GTX100235 IHC-P Image

NQO1 antibody [C2C3], C-term detects NQO1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NQO1 stained by NQO1 antibody [C2C3], C-term (GTX100235) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NQO1 antibody [C2C3], C-term used in Western Blot (WB). GTX100235

GTX100235 WB Image

NQO1 antibody [C2C3], C-term detects NQO1 protein by western blot analysis.
A. 50 μg mouse heart lysate/extract
12% SDS-PAGE
NQO1 antibody [C2C3], C-term (GTX100235) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NQO1 antibody [C2C3], C-term used in Western Blot (WB). GTX100235

GTX100235 WB Image

NQO1 antibody [C2C3], C-term detects NQO1 protein by western blot analysis.
A. 50 μg rat heart lysate/extract
12% SDS-PAGE
NQO1 antibody [C2C3], C-term (GTX100235) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NQO1 antibody [C2C3], C-term used in Western Blot (WB). GTX100235

GTX100235 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NQO1 antibody [C2C3], C-term (GTX100235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang X et al. J Magn Reson Imaging 2024; Effects of 1.5-9.4 T High Static Magnetic Fields on Alcoholic Liver Disease in Mice
Lu YU et al. Oncol Res 2023; 31 (3) : 239-253 PPARα activator irbesartan suppresses the proliferation of endometrial carcinoma cells via SREBP1 and ARID1A.
Lai W et al. Nanomaterials (Basel) 2022; 12 (22) Bacterial Magnetosomes Release Iron Ions and Induce Regulation of Iron Homeostasis in Endothelial Cells.
Application : WB
Reactivity : Human
Ren J et al. Acta Biochim Biophys Sin (Shanghai) 2022; 54 (1) : 77-90 MEF2C ameliorates learning, memory, and molecular pathological changes in Alzheimer’s disease in vivo and in vitro.
Application : WB
Reactivity : Mouse
Ting-Yu Chao et al. Food Sci Nutr 2021; 9 (9) : 4758-4769 Bracteanolide A abrogates oxidative stress-induced cellular damage and protects against hepatic ischemia and reperfusion injury in rats.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Wang X et al. J Magn Reson Imaging 2024; Effects of 1.5-9.4 T High Static Magnetic Fields on Alcoholic Liver Disease in Mice
Lu YU et al. Oncol Res 2023; 31 (3):239-253 PPARα activator irbesartan suppresses the proliferation of endometrial carcinoma cells via SREBP1 and ARID1A.
Lai W et al. Nanomaterials (Basel) 2022; 12 (22) Bacterial Magnetosomes Release Iron Ions and Induce Regulation of Iron Homeostasis in Endothelial Cells.
Application : WB
Reactivity : Human
Ren J et al. Acta Biochim Biophys Sin (Shanghai) 2022; 54 (1):77-90 MEF2C ameliorates learning, memory, and molecular pathological changes in Alzheimer’s disease in vivo and in vitro.
Application : WB
Reactivity : Mouse
Ting-Yu Chao et al. Food Sci Nutr 2021; 9 (9):4758-4769 Bracteanolide A abrogates oxidative stress-induced cellular damage and protects against hepatic ischemia and reperfusion injury in rats.
Application : WB
Reactivity : Human
Hori Y et al. Cells 2021; 10 (5) Aromatic-Turmerone Analogs Protect Dopaminergic Neurons in Midbrain Slice Cultures through Their Neuroprotective Activities.
Application : WB
Reactivity : Mouse
Kuang Y et al. Mol Med Rep 2020; 22 (4):2783-2790 Protective effect of dimethyl fumarate on oxidative damage and signaling in cardiomyocytes.
Application : WB
Reactivity : Rat
Lin DS et al. Cells 2020; 9 (5) Impairment of Proteasome and Autophagy Underlying the Pathogenesis of Leukodystrophy.
Application : WB
Reactivity : Mouse
Huang SY et al. Biomolecules 2019; 9 (8) The Protective Effect of Hispidin against Hydrogen Peroxide-Induced Oxidative Stress in ARPE-19 Cells via Nrf2 Signaling Pathway.
Application : WB
Reactivity : Human
Wu CT et al. Oxid Med Cell Longev 2019; 2019:9056845 Salvianolic Acid C against Acetaminophen-Induced Acute Liver Injury by Attenuating Inflammation, Oxidative Stress, and Apoptosis through Inhibition of the Keap1/Nrf2/HO-1 Signaling.
Application : WB
Reactivity : Mouse
Cai J et al. Cell Rep 2018; 25 (7):1708-1717.e5 autophagy ablation in adipocytes induces * resistance and reveals roles for lipid peroxide and nrf2 signaling in adipose-liver crosstalk.
Application : WB
Reactivity : Mouse
Shin D et al. Free Radic Biol Med 2018; 129:454-462 Nrf2 inhibition reverses resistance to GPX4 inhibitor-induced ferroptosis in head and neck cancer.
Application : WB
Reactivity : Human
Huang KF et al. J Cell Physiol 2018; (Epub) A new copper ionophore DPMQ protects cells against ultraviolet B irradiation by inhibiting the TRPV1 channel.
Application : WB
Reactivity : Human
Ishitsuka Y et al. J Invest Dermatol 2016; 136 (8):1656-63 Lce1 Family Members Are Nrf2-Target Genes that Are Induced to Compensate for the Loss of Loricrin.
Application : WB
Reactivity : Mouse
Lin PL et al. Free Radic Biol Med 2016; 95:121-132 Cytoplasmic localization of Nrf2 promotes colorectal cancer with more aggressive tumors via upregulation of PSMD4.
Application : WB, IHC-P
Reactivity : Monkey
Rachakonda V et al. Sci Rep 2015; 5:14110 M1 Muscarinic Receptor Deficiency Attenuates Azoxymethane-Induced Chronic Liver Injury in Mice.
Application : IHC-P
Reactivity : Mouse
Yeh CH et al. J Cell Physiol 2015; Baicalein decreases hydrogen peroxide-induced damage to NG108-15 cells via upregulation of Nrf2.
Application : WB
Reactivity : Mouse
Liu XM et al. Am J Physiol Heart Circ Physiol 2013; 304 (12):H1634-43 Physiological cyclic strain promotes endothelial cell survival via the induction of heme oxygenase-1.
Application : WB
Reactivity : Human
Chen PH et al. Mol Pharmacol 2013; 83 (3):605-12 Aryl hydrocarbon receptor is a target of 17-Allylamino-17-demethoxygeldanamycin and enhances its anticancer activity in lung adenocarcinoma cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169