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NRF1 antibody

Orthogonal
Anti-NRF1 antibody used in Immunoprecipitation (IP). GTX103179
Anti-NRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103179
Anti-NRF1 antibody used in Western Blot (WB). GTX103179
Anti-NRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103179
Anti-NRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103179
Anti-NRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103179
Anti-NRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103179
Anti-NRF1 antibody used in Western Blot (WB). GTX103179

Cat. No. GTX103179

Reference 12

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NRF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T nuclear , A431 nuclear , HeLa nuclear , HepG2 nuclear

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NRF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168915

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear respiratory factor 1 , ALPHA-PAL

Cellular Localization

Nucleus

Background

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NRF1 antibody used in Immunoprecipitation (IP). GTX103179

GTX103179 IP Image

NRF1 antibody immunoprecipitates NRF1 protein in IP experiments.
IP samples: 293T whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of NRF1 protein by 4 μg NRF1 antibody (GTX103179)
10 % SDS-PAGE
The immunoprecipitated NRF1 protein was detected by NRF1 antibody (GTX103179) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-NRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103179

GTX103179 ICC/IF Image

NRF1 antibody detects NRF1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF1 stained by NRF1 antibody (GTX103179) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

Anti-NRF1 antibody used in Western Blot (WB). GTX103179

GTX103179 WB Image

Orthogonal

NRF1 antibody detects NRF1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg 293T nuclear lysate/extract
C. 30 μg A431 whole cell lysate/extract |
D. 30 μg A431 nuclear lysate/extract
E. 30 μg HeLa whole cell lysate/extract
F. 30 μg HeLa nuclear lysate/extract
G. 30 μg HepG2 whole cell lysate/extract
H. 30 μg HepG2 nuclear lysate/extract
10 % SDS-PAGE
NRF1 antibody (GTX103179) dilution: 1:1000

Anti-NRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103179

GTX103179 IHC-P Image

NRF1 antibody detects NRF1 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
NRF1 antibody (GTX103179) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103179

GTX103179 IHC-P Image

NRF1 antibody detects NRF1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
NRF1 stained by NRF1 antibody (GTX103179) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103179

GTX103179 IHC-P Image

NRF1 antibody detects NRF1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
NRF1 antibody (GTX103179) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103179

GTX103179 IHC-P Image

NRF1 antibody detects NRF1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
NRF1 antibody (GTX103179) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NRF1 antibody used in Western Blot (WB). GTX103179

GTX103179 WB Image

Orthogonal

The data was published in the journal Nat Commun in 2019. PMID: 30833558

REFERENCE

Application Reference

Liang MZ et al. Cell Biosci 2023; 13 (1) : 96 Timely expression of PGAM5 and its cleavage control mitochondrial homeostasis during neurite re-growth after traumatic brain injury.
Mesquita PHC et al. bioRxiv 2023; Resistance Training Diminishes Mitochondrial Adaptations to Subsequent Endurance Training.
Mesquita PHC et al. Physiol Rep 2022; 10 (24) : e15542 Effects of aging and long-term physical activity on mitochondrial physiology and redox state of the cortex and cerebellum of female rats.
Jia-Wei Chen et al. Front Cell Neurosci 2022; 16 : 803718 mito-TEMPO Attenuates Oxidative Stress and Mitochondrial Dysfunction in Noise-Induced Hearing Loss via Maintaining TFAM-mtDNA Interaction and Mitochondrial Biogenesis.
Application : WB
Reactivity : Rat
Mesquita PHC et al. Physiol Rep 2020; 8 (15) : e14526 Acute and chronic effects of resistance training on skeletal muscle markers of mitochondrial remodeling in older adults.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Liang MZ et al. Cell Biosci 2023; 13 (1):96 Timely expression of PGAM5 and its cleavage control mitochondrial homeostasis during neurite re-growth after traumatic brain injury.
Mesquita PHC et al. bioRxiv 2023; Resistance Training Diminishes Mitochondrial Adaptations to Subsequent Endurance Training.
Mesquita PHC et al. Physiol Rep 2022; 10 (24):e15542 Effects of aging and long-term physical activity on mitochondrial physiology and redox state of the cortex and cerebellum of female rats.
Jia-Wei Chen et al. Front Cell Neurosci 2022; 16:803718 mito-TEMPO Attenuates Oxidative Stress and Mitochondrial Dysfunction in Noise-Induced Hearing Loss via Maintaining TFAM-mtDNA Interaction and Mitochondrial Biogenesis.
Application : WB
Reactivity : Rat
Mesquita PHC et al. Physiol Rep 2020; 8 (15):e14526 Acute and chronic effects of resistance training on skeletal muscle markers of mitochondrial remodeling in older adults.
Application : WB
Reactivity : Human
Ma B et al. Nat Commun 2019; 10 (1):1034 The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression.
Lee CT et al. J Steroid Biochem Mol Biol 2018; (Epub) 1,25-dihydroxyvitamin d3 modulates the effects of sublethal bpa on mitochondrial function via activating pi3k-akt pathway and 17β-* secretion in rat granulosa cells.
Application : WB
Reactivity : Rat
Zhang Y et al. Am J Physiol Regul Integr Comp Physiol 2017;:ajpregu.00176.2017 A mitohormetic response to pro-oxidant exposure in the house mouse.
Application : WB
Reactivity : Mouse
Chen YH et al. PLoS One 2016; 11 (4):e0154210 Caveolin-1 Expression Ameliorates Nephrotic Damage in a Rabbit Model of Cholesterol-Induced Hypercholesterolemia.
Application : WB, IHC-P
Chiang MC et al. Exp Cell Res 2015; 338 (2):183-93 Rosiglitazone activation of PPARγ-dependent signaling is neuroprotective in mutant huntingtin expressing cells.
Application : WB
Reactivity : Mouse
Chen YH et al. PLoS One 2014; 9 (1):e71862 Caveolin-1 provides palliation for adverse hepatic reactions in hypercholesterolemic rabbits.
Application : WB, IHC-P
Liu XM et al. Am J Physiol Heart Circ Physiol 2013; 304 (12):H1634-43 Physiological cyclic strain promotes endothelial cell survival via the induction of heme oxygenase-1.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169