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NRF2 antibody [HL1021]

Recombinant antibody Comparison Orthogonal Overexpression
Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826
Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826
Anti-NRF2 antibody [HL1021] used in IHC (Paraffin sections) (IHC-P). GTX635826
Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826
Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826
Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826
Anti-NRF2 antibody [HL1021] used in IHC (Paraffin sections) (IHC-P). GTX635826
Anti-NRF2 antibody [HL1021] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635826

Cat. No. GTX635826

Reference 4

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1021

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, PLA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NRF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909905

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor, erythroid 2 like 2 , HEBP1 , IMDDHH , NRF2

Cellular Localization

Cytoplasm, cytosol,Nucleus

Background

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [HL1021] used in IHC (Paraffin sections) (IHC-P). GTX635826

GTX635826 IHC-P Image

NRF2 antibody [HL1021] detects NRF2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Orthogonal

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Orthogonal

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [HL1021] used in IHC (Paraffin sections) (IHC-P). GTX635826

GTX635826 IHC-P Image

NRF2 antibody [HL1021] detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NRF2 antibody [HL1021] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635826

GTX635826 ICC/IF Image

Orthogonal

NRF2 antibody [HL1021] detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Zhang W et al. Oxid Med Cell Longev 2023; 2023 : 9966355 Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3.
Application : WB
Reactivity : Mouse, Rat
Feng C et al. Cells 2022; 11 (3) Static Magnetic Fields Reduce Oxidative Stress to Improve Wound Healing and Alleviate Diabetic Complications.
Application : WB
Reactivity : Mouse
Lin CY et al. Antioxidant 2021; 10 (12) : 1898 Glucose Activates Lysine-Specific Demethylase 1 through the KEAP1/p62 Pathway.
Application : PLA
Reactivity : Human
Eslami M et al. Cell J 2021; 23 (3) : 319-328 Astaxanthin Protects Human Granulosa Cells against Oxidative Stress through Activation of NRF2/ARE Pathway and Its Downstream Phase II Enzymes.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations