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NRF2 antibody [HL1021]

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826
Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826
Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826
Anti-NRF2 antibody [HL1021] used in IHC (Paraffin sections) (IHC-P). GTX635826
Anti-NRF2 antibody [HL1021] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635826

Cat No. GTX635826

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1021

Isotype

IgG

Application

WB, ICC/IF, IHC-P, PLA

Reactivity

Human, Mouse, Rat
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909905

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear factor, erythroid 2 like 2 , HEBP1 , IMDDHH , NRF2

Cellular Localization

Cytoplasm, cytosol,Nucleus

Background

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [HL1021] used in IHC (Paraffin sections) (IHC-P). GTX635826

GTX635826 IHC-P Image

NRF2 antibody [HL1021] detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NRF2 antibody [HL1021] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635826

GTX635826 ICC/IF Image

NRF2 antibody [HL1021] detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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Package List Price ($)
$ 369
$ 169
Custom Conjugations