Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NRP2 antibody [N2N3] (GTX102397) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.