Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NSD2 antibody [N1], N-term (GTX106306) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using NSD2(GTX106306) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min