NSE antibody [N1C1]

Cat. No. GTX101553
2
Cat. No.  GTX101553
Orthogonal
Anti-NSE antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX101553
GTX101553 IHC-Fr Image

NSE antibody [N1C1] detects NSE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: NSE protein stained by NSE antibody [N1C1] (GTX101553) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
GTX101553 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [N1C1] (GTX101553) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
GTX101553 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX101553 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
GTX101553 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [N1C1] (GTX101553) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-NSE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101553
GTX101553 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ENO2(GTX101553) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
GTX101553 WB Image
Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 27034167

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Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
GTX101553 WB Image

The data was published in the journal Oncotarget in 2016.PMID: 27034167

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Anti-NSE antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
Anti-NSE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
This NSE antibody [N1C1] (GTX101553) is a rabbit polyclonal antibody validated for detection of NSE by WB, IHC-P, and IHC-Fr. Reactivity has been shown in Human, Mouse, and Rat.
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB IHC-P IHC-Fr
  • Reactivity
    Human, Mouse, Rat
Summary
NSE is a 78 kDa gamma-homodimer expressed as a dominant enolase-isoenzyme in neuronal and neuroendocrine tissues. Elevated levels of serum NSE are often associated with neuronal disorders and neural crest-derived tumors. Up to 70 percent of patients with small cell lung carcinoma (SCLC) have high levels of serum NSE, as SCLC cells can express neuronal gene programs.
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