GeneTex
United States (US)

NSE antibody [N1C1]

GTX101553_39721_20170310_IHC-Fr_R_19011506_326.jpg
GTX101553_39721_WB_M_Brain_19011506_357.jpg
GTX101553_39721_IHC_19011506_206.jpg
GTX101553_WB_19011506_149.jpg

Cat No. GTX101553

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENO2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Enolase 2,Hel-S-279,Nse,Eno2

Cellular Localization

Cytoplasm , Cell membrane

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101553_39721_20170310_IHC-Fr_R_19011506_326.jpg

GTX101553 IHC-Fr Image

NSE antibody [N1C1] detects NSE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: NSE protein stained by NSE antibody [N1C1] (GTX101553) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101553_39721_WB_M_Brain_19011506_357.jpg

GTX101553 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX101553 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101553_39721_IHC_19011506_206.jpg

GTX101553 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ENO2(GTX101553) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101553_WB_19011506_149.jpg

GTX101553 WB Image

Sample(30 µg of whole cell lysate)
A:A431(GTX27909)
B:H1299
10% SDS PAGE
GTX101553 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169