We do not recommend use of this product for Tetrahymena samples.
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 50% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant GST-fused human Nup98 (amino acids 1-466)
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
nucleoporin 98 , ADIR2 , NUP196 , NUP96
Cellular Localization
nucleoplasm
Background
Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. [provided by RefSeq, Feb 2016]
WB analysis of S. cereviciae cell extracts using GTX00697 NUP98 antibody [2H10]. Five protein bands identified by this antibody correspond to the sizes of 5 kinds of nucleoporins with multiple GFLG motifs in S. pombe. Dilution : 2 μg/ml
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GTX00697 ICC/IF Image
ICC/IF analysis of S. cereviciae cells using GTX00697 NUP98 antibody [2H10]. Dilution : 10 μg/ml Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min Permeabilization : 1% Triton X-100 for 1 min
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GTX00697 ICC/IF Image
ICC/IF analysis of S. pombe cells using GTX00697 NUP98 antibody [2H10]. Dilution : 10 μg/ml Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min Permeabilization : 1% Triton X-100 for 1 min
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GTX00697 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies. Green : Primary antibody Violet : DAPI Dilution : 0.5 μg/ml Fixation : Cold Methanol (-30 degree C) for 30 min
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GTX00697 ICC/IF Image
ICC/IF analysis of rat neuron cells using GTX00697 NUP98 antibody [2H10]. The dots are Nup98.
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GTX00697 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10]. Dilution : 0.5 μg/ml Fixation : Cold Methanol (-30 degree C) for 30 min
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GTX00697 WB Image
WB analysis of S. pombe cell extracts expressing wilde type Nup98 (Lane 1) or Nup98-GFP protein (Lane 2) using GTX00697 NUP98 antibody [2H10]. Dilution : 1 μg/ml
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GTX00697 WB Image
WB analysis of HeLa nuclear membrane extract using GTX00697 NUP98 antibody [2H10]. Dilution : 1:2000
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GTX00697 Image
Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.