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NUP98 antibody [2H10]

Comparison
Anti-NUP98 antibody [2H10] used in Western Blot (WB). GTX00697
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
Anti-NUP98 antibody [2H10] used in Western Blot (WB). GTX00697
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
Anti-NUP98 antibody [2H10] used in (). GTX00697
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
Anti-NUP98 antibody [2H10] used in Western Blot (WB). GTX00697
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697

Cat. No. GTX00697

Reference 3

Host

 Rat

Clonality

 Monoclonal

Clone Name

 2H10

Isotype

 IgG2c

Application

 WB, ICC/IF, Dot, ELISA

Reactivity

 Human, Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe
Package
100 μg ($399)
See all NUP98 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Dot Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

198 kDa. ( Note )

Product Note

We do not recommend use of this product for Tetrahymena samples.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant GST-fused human Nup98 (amino acids 1-466)

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleoporin 98 , ADIR2 , NUP196 , NUP96

Cellular Localization

nucleoplasm

Background

Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-NUP98 antibody [2H10] used in Western Blot (WB). GTX00697

GTX00697 WB Image

WB analysis of HeLa nuclear membrane extract using GTX00697 NUP98 antibody [2H10].
Dilution : 1:2000

Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697

GTX00697 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10].
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min

Anti-NUP98 antibody [2H10] used in Western Blot (WB). GTX00697

GTX00697 WB Image

WB analysis of S. pombe cell extracts expressing wilde type Nup98 (Lane 1) or Nup98-GFP protein (Lane 2) using GTX00697 NUP98 antibody [2H10].
Dilution : 1 μg/ml

Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697

GTX00697 ICC/IF Image

ICC/IF analysis of rat neuron cells using GTX00697 NUP98 antibody [2H10].
The dots are Nup98.

Anti-NUP98 antibody [2H10] used in (). GTX00697

GTX00697 Image

Comparison

Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.

Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697

GTX00697 ICC/IF Image

ICC/IF analysis of S. cereviciae cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min

Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697

GTX00697 ICC/IF Image

Comparison

ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min

Anti-NUP98 antibody [2H10] used in Western Blot (WB). GTX00697

GTX00697 WB Image

WB analysis of S. cereviciae cell extracts using GTX00697 NUP98 antibody [2H10].
Five protein bands identified by this antibody correspond to the sizes of 5 kinds of nucleoporins with multiple GFLG motifs in S. pombe.
Dilution : 2 μg/ml

Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697

GTX00697 ICC/IF Image

ICC/IF analysis of S. pombe cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min

REFERENCE

Application Reference

Chandra B et al. Cancer Discov 2022; 12 (4) : 1152-1169 Phase Separation Mediates NUP98 Fusion Oncoprotein Leukemic Transformation.
Application : ICC/IF
Reactivity : Human

Clone Reference

Iwamoto M et al. Monoclon Antib Immunodiagn Immunother. 2013; 32 (2):81-90 Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans.
Application : WB, ICC/IF
Fukuhara T et al. Hybridoma (Larchmt). 2005; 24 (5):244-7 Specific monoclonal antibody against the nuclear pore complex protein, nup98.
Application : WB, ICC/IF
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399