APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7.4), 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant human NUP98 protein fragments expressed in E.coli.
Purification
Affinity purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
nucleoporin 98 , ADIR2 , NUP196 , NUP96
Cellular Localization
nucleoplasm
Background
Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
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GTX49240 IP Image
IP analysis of HeLa lysates using control IgG and NUP98 antibody. The precipitates were detected by the same NUP98 antibody.
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GTX49240 WB Image
WB analysis of HeLa, Jurkat and MCF7 lysates using NUP98 antibody at a dilution of 1:1,000.
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REFERENCE
There are currently no references for NUP98 antibody [3B8-D7-H10] (GTX49240). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NUP98 antibody [3B8-D7-H10] (GTX49240). Be the first to share your experience with this product.